MULTIPLE AUTO-ANTIGEN BINDING CAPABILITIES OF MOUSE MONOCLONAL-ANTIBODIES SELECTED FOR RHEUMATOID-FACTOR ACTIVITY
MULTIPLE AUTO-ANTIGEN BINDING CAPABILITIES OF MOUSE MONOCLONAL-ANTIBODIES SELECTED FOR RHEUMATOID-FACTOR ACTIVITY
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DOI：
10.1084/jem.159.5.1429
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发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
THEOFILOPOULOS, AN
中科院分区：
文献类型：
--
作者：
RUBIN, RL;BALDERAS, RS;THEOFILOPOULOS, AN
约1/4的单克隆类风湿因子产生的杂交瘤衍生自融合的脾细胞从MRL/lpr/lpr小鼠与系统性红斑狼疮（SLE）和关节炎表现出多种反应性与其他自身抗原，包括dDNA [变性DNA]，组蛋白和/或细胞质-细胞质元素。这些克隆中的大多数的反应性模式不同，表明每个克隆都有一个单独的B细胞祖先。洗脱抗体的研究表明，单一种类的抗体分子是观察到的多种反应性的原因。抑制实验表明，抗体结合位点可能足够大，以容纳不同的表位。这些发现可能提供进一步的见解抗体多样性的产生和程度，以及系统性自身免疫性疾病的发病机制。
About 1/4 of monoclonal rheumatoid factors produced by hybridomas derived from fusions of spleen cells from MRL/lpr/lpr mice with systemic lupus erythematosus (SLE) and arthritis exhibited multiple reactivities with other autoantigens, including dDNA [denatured DNA], histones and/or cytoskeletal-cytoplasmic elements. The patterns of reactivities of most of these clones differed, indicating that each had a separate B cell ancestor. Studies with eluted antibodies demonstrated that a single species of antibody molecules was responsible for the observed multiple reactivities. Inhibition experiments suggested that an antibody combining site may be large enough to accommodate dissimilar epitopes. These findings may provide further insights into the generation and extent of antibody diverisity as well as the etiopathogenesis of systemic autoimmune diseases.