Temperature responses of soil ammonia-oxidising archaea depend on pH
Temperature responses of soil ammonia-oxidising archaea depend on pH
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DOI：
10.1016/j.soilbio.2016.12.007
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发表时间：
2017-03-01
影响因子：
9.7
通讯作者：
Prosser, James I.
中科院分区：
文献类型：
--
作者：
Gubry-Rangin, Cecile;Novotnik, Breda;Prosser, James I.
氨氧化古菌（AOA）是一组丰富且分布广泛的土壤微生物，对氮循环过程有显着贡献。土壤 pH 值已被确定为 AOA 多样化的主要驱动因素，但其他环境因素也可能很重要。本研究的目的是确定土壤 pH 值是否也影响土壤中 AOA 活性的温度范围。这是通过在 20、30 或 40 摄氏度下 pH 范围为 3.6-7.5 的土壤培养 30 天期间测定氨化和净硝化速率、AOA 丰度和群落组成来评估的。净硝化作用在 20 或 30 摄氏度时最大，土壤之间的最佳温度存在差异，净硝化作用在 40 摄氏度时检测不到，而矿化作用在 40 摄氏度时最大。在 20 和 30 摄氏度孵育后，AOA 群落组成有所不同，大概是通过具有不同最佳温度的种群的选择和生长，而在 40 摄氏度时，则是由于细胞死亡。使用amoA和16S rRNA基因估计的奇古菌细胞丰度和产量之间没有显着关系，可能是由于非氨氧化奇古菌的16S rRNA基因扩增所致。然而，在大多数酸性土壤中，amoA 基因丰度和产量在 20 摄氏度时高于 30 摄氏度，而在中性土壤中则相反。这与嗜中性土壤 AOA 而非嗜酸土壤 AOA 的培养低温最佳温度一致。结果表明，pH 驱动的多样化可能会对 AOA 生理学的其他方面产生影响，包括环境中生长和活动的最佳温度。 (C) 2016 Elsevier Ltd. 保留所有权利。
Ammonia oxidising archaea (AOA) are an abundant and ubiquitously distributed group of soil microorganisms and contribute significantly to nitrogen cycling processes. Soil pH has been identified as a major driver of AOA diversification, but other environmental factors may also be important. The aim of this study was to determine whether soil pH also influenced the temperature range of AOA activity in soil. This was assessed by determining rates of ammonification and net nitrification, and AOA abundance and community composition during incubation of soils with pH in the range 3.6-7.5 at 20, 30 or 40 degrees C for 30 days. Net nitrification was greatest at 20 or 30 degrees C, with variation in optimal temperature between soils, net nitrification was not detectable at 40 degrees C, and mineralisation was greatest at 40 degrees C. AOA community composition differed following incubation at 20 and 30 degrees C, presumably through Selection and growth of populations with different temperature optima, and, at 40 degrees C, due to cell death. There was no significant relationship between thaumarchaeotal cell abundance and yield estimated using amoA and 16S rRNA genes, possibly due to amplification of 16S rRNA genes of non-ammonia-oxidising Thaumarchaeota. However, amoA gene abundance and yield were greater at 20 degrees C than 30 degrees C in the most acidic soils, with the opposite relationship for the most neutral soils. This is consistent with cultivated lower temperature optima for neutrophilic, rather than acidophilic soil AOA. The results indicate that pH driven diversification may have consequences for other aspects of AOA physiology including temperature optima for growth and activity in the environment. (C) 2016 Elsevier Ltd. All rights reserved.