An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming
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DOI：
10.1038/s41467-018-07119-8
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发表时间：
2018-11-22
影响因子：
16.6
通讯作者：
Gadella, Bart M.
中科院分区：
文献类型：
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作者：
Ferraz, Marcia A. M. M.;Rho, Hoon Suk;Gadella, Bart M.
全世界有500多万儿童使用辅助生殖技术受孕，研究集中在增加持续怀孕的可能性上。然而，使用动物模型进行的研究表明，体外胚胎培养对后代发育和健康有不良影响。在体内，输卵管有一段时间，在这段时间里，早期胚胎经历了其(Epi)基因组的完全重新编程，为重新获得(Epi)遗传标记做准备。我们设计了一个输卵管芯片平台，以更好地研究与(外周)基因重编程相关的机制，以及它们在体外和体内胚胎之间的差异程度。与传统的试管受精相比，该设备支持更多的生理性(活体)受精卵基因重新编程。这种方法将有助于识别和研究对受精和植入前发育至关重要的因素，从而提高体外受精受精卵的质量和(表观)遗传完整性，这可能与早期胚胎和后来的胎儿发育有关。
Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi) genome in preparation for the reacquisition of (epi) genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi) genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and preimplantation development, which could improve the quality and (epi) genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development.