Connecting Environment and Genome Plasticity in the Characterization of Transformation-Induced SOS Regulation and Carbon Catabolite Control of the Vibrio cholerae Integron Integrase
Connecting Environment and Genome Plasticity in the Characterization of Transformation-Induced SOS Regulation and Carbon Catabolite Control of the Vibrio cholerae Integron Integrase
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DOI：
10.1128/jb.05982-11
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发表时间：
2012-04-01
影响因子：
3.2
通讯作者：
Mazel, Didier
中科院分区：
文献类型：
--
作者：
Baharoglu, Zeynep;Krin, Evelyne;Mazel, Didier
人类病原体霍乱弧菌携带染色体超整合子（SI）。SI包含串联组织的数百个基因盒的阵列，这些基因盒在没有对细菌施加特定应力的条件下（例如在实验室生长期间）是稳定的。这些盒的重排由相关整合子整合酶的活性催化。了解整合酶表达的调节对于充分理解这种遗传库对细菌适应所起的作用及其与抗生素耐药性发展的联系至关重要。我们以前的工作确定了整合酶是由细菌SOS反应调节的，并且在细菌接合过程中被诱导。在这里，我们表明，转化，另一个水平基因转移（HGT）机制，也触发整合酶的表达，通过SOS诱导，强调HGT在基因组可塑性的重要性。此外，我们报告了一个新的环腺苷酸（cAMP）-cAMP受体蛋白（CRP）依赖的整合酶的调节机制，突出了细胞外环境对染色体基因含量的影响。总之，我们的数据表明，不同的应激反应和调节途径之间的相互作用，重组酶表达的调制，从而显示如何SI重塑机制合并到细菌生理学。
The human pathogen Vibrio cholerae carries a chromosomal superintegron (SI). The SI contains an array of hundreds of gene cassettes organized in tandem which are stable under conditions when no particular stress is applied to bacteria (such as during laboratory growth). Rearrangements of these cassettes are catalyzed by the activity of the associated integron integrase. Understanding the regulation of integrase expression is pivotal to fully comprehending the role played by this genetic reservoir for bacterial adaptation and its connection with the development of antibiotic resistance. Our previous work established that the integrase is regulated by the bacterial SOS response and that it is induced during bacterial conjugation. Here, we show that transformation, another horizontal gene transfer (HGT) mechanism, also triggers integrase expression through SOS induction, underlining the importance of HGT in genome plasticity. Moreover, we report a new cyclic AMP (cAMP)-cAMP receptor protein (CRP)-dependent regulation mechanism of the integrase, highlighting the influence of the extracellular environment on chromosomal gene content. Altogether, our data suggest an interplay between different stress responses and regulatory pathways for the modulation of the recombinase expression, thus showing how the SI remodeling mechanism is merged into bacterial physiology.