Blockade of excitation reveals inhibition of dentate spiny hilar neurons recorded in rat hippocampal slices.
Blockade of excitation reveals inhibition of dentate spiny hilar neurons recorded in rat hippocampal slices.
复制标题
兴奋阻断揭示了大鼠海马切片中记录的齿状棘肺门神经元的抑制。
DOI：
10.1152/jn.1992.68.3.978
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发表时间：
1992
影响因子：
2.5
通讯作者：
Scharfman,HE
中科院分区：
文献类型：
--
作者：
Scharfman,HE
1.在大鼠海马脑片的细胞外和细胞内的记录被用来比较突触反应的穿通路径刺激的颗粒细胞的齿状回，多刺的“苔藓”细胞的门，和区域CA 3c的海马锥体细胞。具体来说，我们问的方面，当地的电路是否可以解释相对脆弱的多刺门神经元的各种侮辱海马。2.棘状门细胞表现出令人惊讶的缺乏抑制后，穿孔路径激活，尽管强大的配对脉冲抑制和抑制性突触后电位（IPSPs）在相邻的颗粒细胞和区域CA 3c锥体细胞在同一切片中响应于相同的刺激。然而，当切片灌注兴奋性氨基酸拮抗剂[6-氰基-7-硝基-喹喔啉-2，3-二酮（CNQX），或CNQX与2-氨基-5-膦酰基戊酸（APV）]，IPSPs可以观察到在多刺门细胞响应于穿孔路径刺激。3.在CNQX的存在下，在棘状门细胞中诱发的IPSPs在其逆转电位和荷包牡丹碱的敏感性方面与在CNQX不存在下在齿状颗粒细胞或海马锥体细胞中记录的IPSPs相似。4.这些结果表明，至少在切片，穿孔路径刺激的棘状门细胞主要是兴奋性的，当兴奋被阻断，潜在的抑制可以揭示。这与齿状回和海马主细胞的情况形成对比，齿状回和海马主细胞通常由抑制支配，只有当抑制受到损害时，才能充分感受到兴奋。（250字处删节）
1. Extracellular and intracellular recordings in rat hippocampal slices were used to compare the synaptic responses to perforant path stimulation of granule cells of the dentate gyrus, spiny “mossy” cells of the hilus, and area CA3c pyramidal cells of hippocampus. Specifically, we asked whether aspects of the local circuitry could explain the relative vulnerability of spiny hilar neurons to various insults to the hippocampus. 2. Spiny hilar cells demonstrated a surprising lack of inhibition after perforant path activation, despite robust paired-pulse inhibition and inhibitory postsynaptic potentials (IPSPs) in adjacent granule cells and area CA3c pyramidal cells in response to the same stimulus in the same slice. However, when the slice was perfused with excitatory amino acid antagonists [6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), or CNQX with 2-amino-5-phosphonovaleric acid (APV)], IPSPs could be observed in spiny hilar cells in response to perforant path stimulation. 3. The IPSPs evoked in spiny hilar cells in the presence of CNQX were similar in their reversal potentials and bicuculline sensitivity to IPSPs recorded in dentate granule cells or hippocampal pyramidal cells in the absence of CNQX. 4. These results demonstrate that, at least in slices, perforant path stimulation of spiny hilar cells is primarily excitatory and, when excitation is blocked, underlying inhibition can be revealed. This contrasts to the situation for dentate and hippocampal principal cells, which are ordinarily dominated by inhibition, and only when inhibition is compromised can the full extent of excitation be appreciated.(ABSTRACT TRUNCATED AT 250 WORDS)