The OsNRAMP1 iron transporter is involved in Cd accumulation in rice.
The OsNRAMP1 iron transporter is involved in Cd accumulation in rice.
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DOI：
10.1093/jxb/err136
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发表时间：
2011-10
影响因子：
6.9
通讯作者：
Nishizawa NK
中科院分区：
文献类型：
--
作者：
Takahashi R;Ishimaru Y;Senoura T;Shimo H;Ishikawa S;Arao T;Nakanishi H;Nishizawa NK
镉（Cd）是一种对人体有害的重金属，尤其是在亚洲，镉在稻米中的积累是一个主要的农业问题。本文研究了水稻铁转运蛋白OsNRAMP1在水稻吸收和转运Cd中的作用。OsNRAMP1：GFP融合蛋白定位于洋葱表皮细胞的质膜上。与用空载体转化的酵母相比，表达OsNRAMP 1的酵母的生长在Cd的存在下受损。此外，表达OsNRAMP1的酵母的Cd含量超过载体对照。OsNRAMP 1在高Cd积累品种（Habataki）中的表达高于低Cd积累品种（Sasanishiki），而OsNRAMP 1的氨基酸序列在Sasanishiki和Habataki之间显示出100%的同一性。过量表达OsNRAMP1增加了水稻叶片中Cd的积累。这些结果表明，OsNRAMP1参与了植物细胞对Cd的吸收和转运，OsNRAMP1在根中的高表达可能导致地上部Cd积累量的增加。以上结果表明OsNRAMP1是水稻高水平镉积累的重要蛋白。
Cadmium (Cd) is a heavy metal toxic to humans and the accumulation of Cd in the rice grain is a major agricultural problem, particularly in Asia. The role of the iron transporter OsNRAMP1 in Cd uptake and transport in rice was investigated here. An OsNRAMP1:GFP fusion protein was localized to the plasma membrane in onion epidermal cells. The growth of yeast expressing OsNRAMP1 was impaired in the presence of Cd compared with yeast transformed with an empty vector. Moreover, the Cd content of OsNRAMP1-expressing yeast exceeded that of the vector control. The expression of OsNRAMP1 in the roots was higher in a high Cd-accumulating cultivar (Habataki) than a low Cd-accumulating cultivar (Sasanishiki) regardless of the presence of Cd, and the amino acid sequence of OsNRAMP1 showed 100% identity between Sasanishiki and Habataki. Over-expression of OsNRAMP1 in rice increased Cd accumulation in the leaves. These results suggest that OsNRAMP1 participates in cellular Cd uptake and Cd transport within plants, and the higher expression of OsNRAMP1 in the roots could lead to an increase in Cd accumulation in the shoots. Our results indicated that OsNRAMP1 is an important protein in high-level Cd accumulation in rice.