A novel BR-SMAD is required for larval development in barber's pole worm Haemonchus contortus.
A novel BR-SMAD is required for larval development in barber's pole worm Haemonchus contortus.
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捻转血矛线虫 (Haemonchus contortus) 幼虫发育需要一种新型 BR-SMAD
DOI：
10.15698/mic2021.02.742
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发表时间：
2020-12-23
期刊：
影响因子：
--
通讯作者：
Hu M
中科院分区：
文献类型：
--
作者：
Li F;Qin P;Ye L;Gupta N;Hu M
SMAD蛋白介导TGF-β信号传导，从而调控后生动物的发育;然而，在小反刍动物常见的吸血寄生线虫-捻转血矛线虫中，SMAD蛋白的定义并不明确。在这里，我们的特点是在H的R-SMAD家族蛋白。称为HcSMA 2的捻转线虫SMA 2（CeSMA 2）与参与骨形态发生蛋白（BMP）信号传导的秀丽隐杆线虫SMA 2密切相关。Hcsma 2在H.但在成年雄性蠕虫中高度诱导。RNA干扰Hcsma 2延缓了感染性L3幼虫向L4幼虫的转变。此外，双分子荧光互补显示了HcSMA 2与TGF-β激活的R SMAD（HcDAF 8）的相互作用。这些结果共同表明，在H.这是幼虫分化所必需的，并强调了寄生蠕虫中BMP信号的适应性功能再利用。
SMAD proteins mediate TGF-β signaling and thereby regulate the metazoan development; however, they are poorly defined in Haemonchus contortus–a common blood-sucking parasitic nematode of small ruminants. Here, we characterized an R-SMAD family protein in H. contortus termed HcSMA2, which is closely related to Caenorhabditis elegans SMA2 (CeSMA2) involved in the bone morphogenetic protein (BMP) signaling. Hcsma2 is transcribed in all developmental stages of H. contortus but highly induced in the adult male worms. The RNA interference with Hcsma2 retarded the transition of infective L3 into L4 larvae. Besides, the bimolecular fluorescence complementation revealed the interaction of HcSMA2 with a TGF-β-activated-R-SMAD (HcDAF8). Together these results show a BMP-like receptor-regulated SMAD in H. contortus that is required for larval differentiation and underscore an adaptive functional repurposing of BMP-signaling in parasitic worms.