Neonatal goitrogen treatment increases adult testis size and sperm production in the mouse.
Neonatal goitrogen treatment increases adult testis size and sperm production in the mouse.
复制标题
DOI：
10.1002/j.1939-4640.1993.tb03261.x
复制
发表时间：
1993-11
影响因子：
--
通讯作者：
Kimberly L. Joyce;John Porcelli;P. Cooke
中科院分区：
文献类型：
--
作者：
Kimberly L. Joyce;John Porcelli;P. Cooke
雄性大鼠甲状腺功能减退在新生儿的生活表现出前所未有的增加成年睾丸大小和每日精子产量（DSP）。为了确定这种效应是否是大鼠独有的，或者也可以在其他物种中得到证明，我们研究了可逆致甲状腺肿剂6-丙基-2-硫氧嘧啶（PTU）对小鼠成年睾丸大小和功能的新生儿治疗效果。雄性Swiss-Webster小鼠未经处理（对照）或从出生到产后第25天通过向其母亲的水中添加0.1%（w/v）给予PTU。然后将所有幼仔断奶，不给予进一步治疗。使用氚化胸苷放射自显影术在0、5、10、15、20和25天时在一些对照和治疗小鼠中检查支持细胞增殖，而其余小鼠在90天时被杀死以确定各种生殖参数。新生儿PTU治疗降低了生长; 4周龄时治疗小鼠的体重比对照组低57%。在停止PTU治疗后，治疗的小鼠迅速生长，尽管它们的体重从未等于对照组，在90日龄时仍小17%。在90日龄时，与对照组相比，PTU处理小鼠的睾丸重量和DSP分别增加约30%和50%。尽管睾丸重量和功能增加，但对照组和给药组小鼠的血清睾酮浓度无差异。给药小鼠的睾丸和附睾组织学与对照组相似，而给药小鼠的附睾精子活动且形态正常。在治疗的小鼠中，支持细胞增殖发生改变。（250字处删节）
Male rats made hypothyroid during neonatal life show unprecedented increases in adult testis size and daily sperm production (DSP). To determine if this effect was unique to the rat or could also be demonstrated in other species, we examined the effects of neonatal treatment with the reversible goitrogen 6-propyl-2-thiouracil (PTU) on adult testis size and function in the mouse. Male Swiss-Webster mice were untreated (control) or given PTU by adding 0.1% (w/v) to their mother's water from birth to day 25 postpartum. All pups were then weaned and given no further treatment. Sertoli cell proliferation was examined using tritiated thymidine autoradiography in some control and treated mice at 0, 5, 10, 15, 20, and 25 days, while the remainder were killed at 90 days to determine a variety of reproductive parameters. Neonatal PTU treatment decreased growth; body weight of treated mice at 4 weeks of age was 57% less than controls. Treated mice grew rapidly following cessation of PTU treatment, although their weights never equalled controls, remaining 17% smaller at 90 days of age. At 90 days of age, testis weight and DSP were increased by approximately 30% and 50%, respectively, in PTU-treated mice compared to controls. Despite the increased testis weight and function, serum testosterone concentrations were not different in control and treated mice. Testicular and epididymal histology in treated mice was similar to controls, while epididymal sperm in treated mice were motile and morphologically normal. Sertoli cell proliferation was altered in treated mice.(ABSTRACT TRUNCATED AT 250 WORDS)