High prevalence anti-Trypanosoma cruzi antibodies, among blood donors in the State of Puebla, a non-endemic area of Mexico
High prevalence anti-Trypanosoma cruzi antibodies, among blood donors in the State of Puebla, a non-endemic area of Mexico
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DOI：
10.1590/s0074-02762002000700004
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发表时间：
2002-10-01
期刊：
影响因子：
--
通讯作者：
Pérez-Fuentes, R
中科院分区：
文献类型：
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作者：
Sánchez-Guillén, MC;Barnabé, C;Pérez-Fuentes, R
在许多拉丁美洲国家，输血是南美锥虫病的第二个最常见的传播途径。在墨西哥，查加斯病的流行率和输注被克氏锥虫污染的血液的影响尚不清楚。我们测定了T.用间接血凝试验（IHA）和酶联免疫吸附试验（ELISA）检测了2140名献血员（男1,423名，女647名，年龄19-65岁）的克氏锥虫病。克氏寄生虫，其遗传特征类似于TBAR/ME/1997/RyD-V1（T. cruzi I）分离自同一地点收集的Triatoma barberi标本。用IHA和ELISA试验的血清阳性率分别高达8.5%和9%，而两种试验共同使用的血清阳性率高达7.7%。我们在墨西哥的非流行区发现了高血清阳性率，与疾病发生的流行国家（如巴西）（0.7%）相当。玻利维亚（13.7%）和阿根廷（3.5%）。在城市地区的样本中观察到的最高值，与持续的农村移民和献血者缺乏控制有关，表明T。克氏病的发病率高于血液感染的发病率，如同一地区的肝炎（0.1%）和艾滋病（0.1%）。
Blood transfusion is the second most common transmission route of Chagas disease in many Latin American countries. In Mexico, the prevalence of Chagas disease and impact of transfusion of Trypanosoma cruzi-contaminated blood is not clear. We determined the seropositivity to T. cruzi in a representative random sample, of 2.140 blood donors (1,423 men and 647 women, aged 19-65 years), from a non-endemic state of almost 5 millions of inhabitants by the indirect hemagglutination (IHA) and enzyme linked immunosorbent assay (ELISA) tests using one autochthonous antigen from T. cruzi parasites, which were genetically characterized like TBAR/ME/1997/RyD-V1 (T. cruzi I) isolated from a Triatoma barberi specimen collected in the same locality. The seropositivity was up to 8.5% and 9% with IHA and ELISA tests, respectively, and up to 7.7% using both tests in common. We found high seroprevalence in a non-endemic area of Mexico, comparable to endemic countries where the disease occurs, e.g. Brazil (0.7%). Bolivia (13.7%) and Argentina (3.5%). The highest values observed in samples from urban areas, associated to continuous rural emigration and the absence of control in blood donors, suggest unsuspected high risk of transmission of T. cruzi, higher than those reported for infections by blood e.g. hepatitis (0.1%) and AIDS (0.1%) in the same region.