Performance and microbial community profiles in pilot-scale biofilter for ammonia, iron and manganese removal at different dissolved oxygen concentrations
Performance and microbial community profiles in pilot-scale biofilter for ammonia, iron and manganese removal at different dissolved oxygen concentrations
复制标题
不同溶解氧浓度下中试规模去除氨、铁和锰的生物过滤器的性能和微生物群落概况
DOI：
10.1007/s11274-019-2617-x
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发表时间：
2019-03-01
影响因子：
4.1
通讯作者：
Zhang, Jie
中科院分区：
文献类型：
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作者：
Cheng, Qingfeng;Huang, Yang;Zhang, Jie
溶解氧（DO）是生物系统中的重要运行参数。在本研究中，构建了一个中试规模的生物过滤器，考察了氨，铁和锰的去除效率，以及微生物群落结构的演变在不同的DO浓度。结果表明，当DO由8 mg/L降至4 mg/L时，铁、锰仍能完全去除，但出水氨氮浓度升高，当DO约为4 mg/L时，氨氮浓度已超过0.5 mg/L的允许值。在DO充足的条件下，氨氮和锰去除的主要功能微生物为亚硝化单胞菌（Nitrosomonas）和腐殖菌（Crenothrix），主要分布在滤床0.8 m和0.8 m处，丰度分别为8.61%和16.87%，铁去除的主要功能微生物为腐殖菌（Crenothrix）和石蒜菌（Gallionellain），丰度分别为30.45%和9.77%。随着溶解氧浓度的降低，铁氧化细菌（IOB、CrenothrixandGallionella）的数量不受影响，而锰氧化细菌（MnOB、Crenothrix）的数量增加，使锰氧化完全。而0.4 m和0.8 m处的氨氧化细菌（AOB、亚硝化球菌）数量随着出水氨氮浓度的增加而明显减少。除此之外，还发现了几种可能与甲烷、硫化氢和有机物去除有关的细菌，此外还发现了少量古菌，如亚硝化球菌和铁浆菌，它们分别能氧化氨和亚铁离子.图示摘要
Dissolved oxygen (DO) is a significant operational parameter in biological systems. In this study, a pilot-scale biofilter was constructed to investigate the removal efficiency of ammonia, iron and manganese, as well as the microbial community structure evolution at different DO concentrations. Results indicated that when DO decreased from 8 to 4 mg/L, iron and manganese were still completely removed, however the concentration of ammonia in the effluent increased, and exceeded the permitted limit of 0.5 mg/L when DO was about 4 mg/L. The main functional microbes for ammonia and manganese removal wereNitrosomonasandCrenothrix, which was mainly distributed at 0.8 and 0.8 m of the filter bed with a corresponding abundance of 8.61% and 16.87% in sufficient DO considition, respectively; while iron was mainly removed byCrenothrixandGallionellain 0 m with a corresponding abundance of 30.45% and 9.77%. With the decreasing of DO concentration, iron oxidizing bacteria (IOB,CrenothrixandGallionella) was not affected, while the abundance of manganese oxidizing bacteria (MnOB,Crenothrix) increased to completely oxidize manganese. However, the amount of ammonia oxidizing bacteria (AOB,Nitrosococcus) at 0.4 and 0.8 m of the filter depth obviously decreased with increased ammonia in the effluent. Kinds of other bacteria which may be related to methane, hydrogen sulfide and organic matter removal, were also found. In addition, small part of archaea was also detected, such asCandidatus NitrososphaeraandFerroplasma, which could oxdize ammonia and ferrous iron, respectively.Graphical Abstract