Isoliquiritigenin suppresses cocaine-induced extracellular dopamine release in rat brain through GABAB receptor
Isoliquiritigenin suppresses cocaine-induced extracellular dopamine release in rat brain through GABAB receptor
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DOI：
10.1016/j.ejphar.2008.03.054
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发表时间：
2008-06-10
影响因子：
5
通讯作者：
Yang, Chae Ha
中科院分区：
文献类型：
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作者：
Jang, Eun Young;Choe, Eun Sang;Yang, Chae Ha
甘草含有多种黄酮类化合物作为主要成分，包括异甘草素、甘草苷、甘草素和甘草皂苷。它具有抗炎、抗癌、抗组织等多种生物活性。由于对药物成瘾知之甚少，我们进行了一项研究，研究甘草及其活性成分异甘草素对运动大鼠急性可卡因诱导的细胞外多巴胺释放的影响。雄性 Sprague-Dawley 大鼠在注射可卡因（20 mg/kg，腹膜内 (i.p.)）前 1 小时口服给予 G. radix 或异甘草素的甲醇提取物。通过体内微透析测量细胞外多巴胺。根提取物和异甘草素以剂量依赖性方式抑制可卡因诱导的伏核细胞外多巴胺水平。异甘草素抑制多巴胺释放导致 c-Fos 表达减弱，c-Fos 是可卡因诱导的早期基因。 GABA(B) 受体拮抗剂完全阻止异甘草素的作用。因此，这些结果表明，G.radix和异甘草素通过调节GABAB受体抑制可卡因诱导的多巴胺释放，表明异甘草素可能有效阻断可卡因的增强作用。 (c) 2008 Elsevier B.V. 保留所有权利。
Glycyrrhizae radix (licorice) comprises a variety of flavonoids as major constituents including isoliquiritigenin, liquiritin, liquiritigenin, and glycyrrihizin. It has shown various biological activities such as anti-inflammatory, anti-carcinogenic and antihistamic. As very little is known in regard to drug addiction, we carried out a study on the effect of G. radix and its active component, isoliquiritigenin, on acute cocaine-induced extracellular dopamine release in moving rats. Male Sprague-Dawley rats were orally administered with methanolic extracts of G. radix or isoliquiritigenin 1 h prior to an injection of cocaine (20 mg/kg, intraperitoneal (i.p.)). Extracellular dopamine was measured by in vivo microdialysis. Extract of G. radix and isoliquiritigenin inhibited cocaine-induced extracellular dopamine level in the nucleus accumbens by dose-dependent manner. Inhibition of dopamine release by isoliquiritigenin resulted in attenuation of the expression of c-Fos, an immediately early gene induced by cocaine. Effect of isoliquiritigenin was completely prevented by a GABA(B) receptor antagonist. Thus, these results showed that G. radix and isoliquiritigenin inhibit cocaine-induced dopamine release by modulating GABAB receptor, suggesting that isoliquiritigenin might be effective in blocking the reinforcing effects of cocaine. (c) 2008 Elsevier B.V. All rights reserved.