NtGRAS1, a novel stress-induced member of the GRAS family in tobacco, localizes to the nucleus
NtGRAS1, a novel stress-induced member of the GRAS family in tobacco, localizes to the nucleus
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DOI：
10.1016/j.jplph.2006.07.010
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发表时间：
2007-09-01
影响因子：
4.3
通讯作者：
Maxwell, Denis P.
中科院分区：
文献类型：
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作者：
Czikkel, Beatrix E.;Maxwell, Denis P.
我们报告了烟草 GRAS 基因家族新成员 NtGRAS1 的分离和初步表征。对预测氨基酸序列的分析表明，NtGRAS1 具有 GRAS 家族所有成员共有的高度保守的羧基末端基序。抗霉素 A、H2O2、水杨酸和 L-半胱氨酸在烟草 (BY-2) 悬浮细胞中强烈诱导 NtGRAS1 表达，这些物质都被发现可以提高细胞内活性氧水平。用一氧化氮供体硝普钠处理细胞也引发了 NtGRAS1 表达的增加。通过使用蛋白激酶和磷酸酶作用的抑制剂，我们发现应激诱导的 NtGRAS1 诱导需要可逆磷酸化，并且活性氧以及 NO 依赖性信号通路可能共享关键的细胞内成分。有趣的是，在土壤生长的植物中，仅在根部发现 NtGRAS1 的高组成型表达，而在抗霉素 A 处理或丁香假单胞菌感染后，叶组织中的白色表达被强烈诱导。 GRAS 家族的许多成员都参与转录调节，我们发现 NtGRAS1-GFP 融合蛋白定位于洋葱表皮细胞的细胞核，这支持了 NtGRAS1 的这种功能。我们的数据表明 NtGRAS1 可能代表参与植物胁迫反应的重要转录调节因子。 (c) 2006 年爱思唯尔有限公司。版权所有。
We report the isolation and initial characterization of a new member of the GRAS gene family from tobacco, NtGRAS1. Analysis of the predicted amino acid sequence shows that NtGRAS1 shares the highly conserved carboxy-terminal motifs common to all members of the GRAS family. NtGRAS1 expression was strongly induced in tobacco (BY-2) suspension cells by antimycin A, H2O2, salicylic acid, and L-cysteine which were all found to raise intracellular reactive oxygen levels. An increase in NtGRAS1 expression was also triggered by treating cells with the nitric oxide donor sodium nitroprusside. By employing inhibitors of protein kinase and phosphatase action, we show that reversible phosphorylation is required for the stress-induced induction of NtGRAS1 and that reactive oxygen as well as NO-dependent signaling pathways probably share key intracellular components. Interestingly, in soil-grown plants, high constitutive expression of NtGRAS1 was found only in roots white expression was strongly induced in leaf tissue upon antimycin A treatment or following Pseudomonas syringae infection. Many members of the GRAS family are implicated in regulating transcription and this function for NtGRAS1 is supported by our finding that an NtGRAS1-GFP fusion protein localizes to the nucleus of onion epidermal cells. Our data suggest that NtGRAS1 may represent an important transcriptional regulator involved in the plant stress response. (c) 2006 Elsevier GmbH. All rights reserved.