Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in Fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis
Cotton AnnGh3 Encoding an Annexin Protein is Preferentially Expressed in Fibers and Promotes Initiation and Elongation of Leaf Trichomes in Transgenic Arabidopsis
复制标题
编码膜联蛋白的棉花 AnnGh3 优先在纤维中表达并促进转基因拟南芥叶毛状体的起始和伸长
DOI：
10.1111/jipb.12063
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发表时间：
2013-10-01
影响因子：
11.4
通讯作者：
Li, Xue-Bao
中科院分区：
文献类型：
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作者：
Li, Bing;Li, Deng-Di;Li, Xue-Bao
膜联蛋白是钙调节磷脂结合蛋白的多家族。为了研究膜联蛋白在纤维发育中的作用，从棉花（Gossypium hirsutum）中分离出编码假定膜联蛋白的四个基因，并命名为 AnnGh3、AnnGh4、AnnGh5 和 AnnGh6。定量逆转录聚合酶链反应（qRT-PCR）结果表明，AnnGh3、AnnGh4和AnnGh5优先在纤维中表达，而AnnGh6的转录本主要在根中积累。在纤维发育过程中，AnnGh3/4/5基因的转录本主要积累在快速伸长的纤维中。随着纤维细胞的进一步发育，其表达活性急剧下降至相对较低的水平。原位杂交结果表明AnnGh3和AnnGh5在起始纤维细胞(0-2DPA)中表达。此外，它们在纤维中的表达也受到植物激素和[Ca2+]的调节。亚细胞定位分析发现AnnGh3蛋白定位于细胞质。 AnnGh3在拟南芥中的过度表达导致转基因植物叶片上毛状体密度和长度显着增加，表明AnnGh3可能参与棉花纤维细胞的起始和伸长。
The annexins are a multifamily of calcium-regulated phospholipid-binding proteins. To investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton (Gossypium hirsutum) and designated AnnGh3, AnnGh4, AnnGh5, and AnnGh6. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results indicated that AnnGh3, AnnGh4, and AnnGh5 were preferentially expressed in fibers, while the transcripts of AnnGh6 were predominantly accumulated in roots. During fiber development, the transcripts of AnnGh3/4/5 genes were mainly accumulated in rapidly elongating fibers. With fiber cells further developed, their expression activity was dramatically declined to a relatively low level. In situ hybridization results indicated that AnnGh3 and AnnGh5 were expressed in initiating fiber cells (0-2 DPA). Additionally, their expression in fibers was also regulated by phytohormones and [Ca2+]. Subcellular localization analysis discovered that AnnGh3 protein was localized in the cytoplasm. Overexpression of AnnGh3 in Arabidopsis resulted in a significant increase in trichome density and length on leaves of the transgenic plants, suggesting that AnnGh3 may be involved in fiber cell initiation and elongation of cotton.