Alterations in expression of murine thymocyte proteins and glycoproteins during fetal thymus development.
Alterations in expression of murine thymocyte proteins and glycoproteins during fetal thymus development.
复制标题
胎儿胸腺发育过程中小鼠胸腺细胞蛋白和糖蛋白表达的变化。
DOI：
10.1016/0161-5890(80)90108-x
复制
发表时间：
1980
影响因子：
3.6
通讯作者：
Gottlieb,PD
中科院分区：
文献类型：
--
作者：
Kamarck,ME;Gottlieb,PD
为了描述T细胞在小鼠胸腺分化和增殖过程中的生化变化，我们比较了妊娠14-19天成年胸腺细胞和胎儿胸腺细胞的蛋白和Con - a结合糖蛋白。用125i和乳酸过氧化物酶标记细胞，制备NP-40提取物，用兔抗小鼠胸腺细胞血清和金黄色葡萄球菌（Cowan I）菌株获得全提取物和免疫沉淀，在还原条件下十二烷基硫酸钠（SDS-PAGE）存在下进行聚丙烯酰胺凝胶电泳。此外，未标记的胎儿和成人胸腺细胞的NP-40提取物进行SDS-PAGE，凝胶与125i标记的Con A反应，然后干燥和放射自显影。结果表明：(1)早期胎儿胸腺细胞t200样成分的表观分子量（200+K）高于晚期胎儿和成年胸腺细胞；(2)携带Thy-1同种异体抗原的T25成分从妊娠早期非常低的数量增加到17-19 d的成年水平；(3)成年胸腺细胞表达表观分子量为12,000的Con - a非反应性表面成分，而胎儿胸腺细胞中这种成分的含量要低得多；(4)胎儿胸腺细胞在妊娠的所有日子里都表达一种Con - a非活性表面成分，其表观分子量为10,000，而在成年胸腺细胞上几乎检测不到（如果有的话）。
To describe the biochemical alterations that accompany T cell differentiation and proliferation in the mouse thymus, proteins and Con A-binding glycoproteins of adult thymocytes and of fetal thymocytes on days 14–19 of gestation were compared. Cells were labelled with125I and lactoperoxidase, NP-40 extracts were prepared, and both whole extracts and immunoprecipitates obtained with rabbit anti-mouse thymocyte serum andStaphylococcus aureus,Cowan I strain, were subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) under reducing conditions. In addition, NP-40 extracts of unlabelled fetal and adult thymocytes were subjected to SDS-PAGE, and the gels reacted with125I-labelled Con A before drying and autoradiography. Results obtained suggest that: (1) T200-like components of early fetal thymocytes have a higher apparent mol. wt(200+K) than those of late fetal and adult cells; (2) the quantity of the T25 component which bears the Thy-1 alloantigen increases from very low early in gestation to adult levels by day 17–19; (3) adult thymocytes express a Con A-unreactive surface component of apparent mol. wt 12,000 which is present in much lower amounts on fetal thymocytes; (4) fetal thymocytes on all days of gestation express a Con A-unreactive surface component of apparent mol. wt 10,000 which is barely (if at all) detectable on adult thymocytes.