Mitotic nuclear pore complex segregation involves Nup2 in Aspergillus nidulans
Mitotic nuclear pore complex segregation involves Nup2 in Aspergillus nidulans
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DOI：
10.1083/jcb.201610019
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发表时间：
2017-09-01
影响因子：
7.8
通讯作者：
Osmani, Stephen A.
中科院分区：
文献类型：
--
作者：
Suresh, Subbulakshmi;Markossian, Sarine;Osmani, Stephen A.
核孔蛋白Nup2通过其导入的α结合域和RAN结合域促进了核孔复合体在相间的转运。然而，在有丝分裂过程中，Nidulans曲霉和脊椎动物Nup2也定位于染色质，提示有丝分裂功能。在这项研究中，我们报告了Nup2是有丝分裂的鼻咽癌遗传所必需的。有趣的是，Nup2在有丝分裂NPC分离过程中的作用独立于其输入素α和RAN结合域，但依赖于定位和生存所必需的中心靶向域。为了测试有丝分裂染色质相关的Nup2是否在分离过程中将NPC与染色质连接起来，我们通过Nup133和组蛋白H1在NPC和染色质之间提供了人工联系。使用这种方法，我们绕过了Nup2对NPC分离的要求。这表明，有丝分裂DNA通过Nup2的有丝分裂特异染色质关联将有丝分裂DNA和NPC分离联系起来，从而确保有丝分裂NPC准确地分离到子核。
Transport through nuclear pore complexes (NPCs) during interphase is facilitated by the nucleoporin Nup2 via its importin a-and Ran-binding domains. However, Aspergillus nidulans and vertebrate Nup2 also locate to chromatin during mitosis, suggestive of mitotic functions. In this study, we report that Nup2 is required for mitotic NPC inheritance in A. nidulans. Interestingly, the role of Nup2 during mitotic NPC segregation is independent of its importin alpha-and Ran-binding domains but relies on a central targeting domain that is necessary for localization and viability. To test whether mitotic chromatin-associated Nup2 might function to bridge NPCs with chromatin during segregation, we provided an artificial link between NPCs and chromatin via Nup133 and histone H1. Using this approach, we bypassed the requirement of Nup2 for NPC segregation. This indicates that A. nidulans cells ensure accurate mitotic NPC segregation to daughter nuclei by linking mitotic DNA and NPC segregation via the mitotic specific chromatin association of Nup2.