Programmed cell death as a prognostic indicator for radiation therapy in cervical carcinoma patients: a pilot study.
Programmed cell death as a prognostic indicator for radiation therapy in cervical carcinoma patients: a pilot study.
复制标题
DOI：
10.4103/0973-1482.16090
复制
发表时间：
2005
影响因子：
1.3
通讯作者：
S. Bhosle;N. Huilgol;K. Mishra
中科院分区：
文献类型：
--
作者：
S. Bhosle;N. Huilgol;K. Mishra
在临床实践中，放射治疗在IIIB期宫颈癌中经常失败，因此需要开发一种预测癌症患者放射治疗预后的方法。我们试图评估第一次分割剂量放射治疗后宫颈癌细胞膜流动性（MF）变化和相关凋亡细胞死亡与IIIB期宫颈癌患者治疗结果的相关性。材料与方法收集15例经病理证实的宫颈癌患者的活检标本，分别在第一次分割放射剂量为2格雷（戈伊）前和后24 h采集。用Dulbecco改良Eagle培养基（DMEM）制备细胞悬液，用荧光偏振法测定MF，TUNEL法测定凋亡指数（AI）。结果宫颈癌细胞经体外照射后，MF和AI明显增加。MF的变化与患者的治疗结果有显著相关性（P <0.1）.结论体外照射后宫颈癌细胞MF发生明显变化，细胞凋亡率明显增加。宫颈癌患者首次分割剂量放疗后AI的变化可作为宫颈癌患者放疗预后的预测指标。
PURPOSE In clinical practice, radiation therapy often fails in cervical carcinoma stage IIIB and there is a need to develop a predictive assay for prognosis of radiation treatment outcome in cancer patient. We have attempted to evaluate the relevance of changes in Membrane Fluidity (MF) and associated apoptotic cell death in cervical cancer cells after first fractionated dose of radiation therapy to treatment outcome of stage IIIB cervical carcinoma patients. MATERIALS AND METHODS Biopsies of 15 patients with histologically proven cervix cancer were collected from the patients before and 24 h after first fractionated radiation dose of 2 grays (Gy). Cell suspension made in Dulbecco's Modified Eagle's Medium (DMEM) were used for further investigations and cell suspension of cervix cancer patient were used to measure MF by fluorescence polarization method and apoptotic index (AI) was determined by Tdt dUTP Nucleotide End Labeling (TUNEL) assay. RESULTS A substantial increase in MF and AI was observed in cervical cancer cells irradiated ex vivo . A significant correlation ( P P > 0.1) was detected between changes in MF and treatment outcome of patients. CONCLUSION Preliminary results showed significant change in MF and a marked increase in percentage apoptosis of cervix cancer cells irradiated ex vivo . The changes in AI after first fractionated dose of radiotherapy in cervical carcinoma patients may provide a predictor of prognosis for radiotherapy in uterine cervical carcinoma patients.