Identification of a specific pattern of downregulation in expression of isoforms of vascular endothelial growth factor in dilated cardiomyopathy
Identification of a specific pattern of downregulation in expression of isoforms of vascular endothelial growth factor in dilated cardiomyopathy
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DOI：
10.1136/heart.88.4.412
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发表时间：
2002-10-01
期刊：
影响因子：
5.7
通讯作者：
Van Veldhuisen, DJ
中科院分区：
文献类型：
--
作者：
De Boer, RA;Henning, RH;Van Veldhuisen, DJ
VEGF 121、VEGF 165和VEGF 189同种型的表达（凝胶上的PCR产物分别为177 bp、312 bp和384 bp）显示在图1A中。GAPDH在对照组（非衰竭或NF）和IDC患者（衰竭或F）中的表达水平相似。图1B显示与对照相比，在衰竭心肌中VEGF 121/GAPDH的cDNA比率显著降低（0.47（0.04）v1.2（0.21）= 40%; p= 0.002）。VEGF 165（2.2（0.13）v2.7（0.24）= 82%; p= 0.27）和VEGF 189（1.6（0.09）v1.89（0.24）= 83%; p= 0.41）表现出类似的趋势。输入模板的量与所有同种型的观察到的信号强相关（p< 0.01）（图1C：VEGF 121; VEGF 165：r2 = 0.98; VEGF 189：r2 = 0.99）。与对照相比，IDC患者中蛋白VEGF/GAPDH比率（图1D，E）降低（1.17（0.18）v0.64（0.08）= 55%; p= 0.01）。临床参数和VEGF表达水平之间没有相关性。
RESULTSThe expression of VEGF 121, VEGF 165, and VEGF 189 isoforms (PCR products of 177 bp, 312 bp, and 384 bp on the gel, respectively) is displayed in fig 1A. GAPDH was expressed at similar levels in controls (non-failing or NF) and in IDC patients (failing or F). Figure 1B shows that cDNA ratio of VEGF 121/GAPDH is significantly reduced in failing myocardium as compared to controls (0.47 (0.04) v 1.2 (0.21)= 40%; p= 0.002). VEGF 165 (2.2 (0.13) v 2.7 (0.24)= 82%; p= 0.27) and VEGF 189 (1.6 (0.09) v 1.89 (0.24)= 83%; p= 0.41) exhibit a similar trend. The amount of input template strongly correlated (p< 0.01) with the observed signals for all isoforms (fig 1C: VEGF 121; VEGF 165: r 2= 0.98; VEGF 189: r 2= 0.99). Protein VEGF/GAPDH ratios (fig 1D, E) are decreased in IDC patients compared to controls (1.17 (0.18) v 0.64 (0.08)= 55%; p= 0.01). No correlations between clinical parameters and VEGF expression levels were established.