A protective immune response in mice to viral components other than hemagglutinin in a live influenza A virus vaccine model
A protective immune response in mice to viral components other than hemagglutinin in a live influenza A virus vaccine model
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DOI：
10.1016/j.vaccine.2003.11.019
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发表时间：
2004-06-02
期刊：
影响因子：
5.5
通讯作者：
Kawaoka, Y
中科院分区：
文献类型：
--
作者：
Horimoto, T;Takada, A;Kawaoka, Y
先前，我们产生了具有嵌合型（A/B）血凝素（HA）的甲型流感病毒，其中A型HA的免疫原性区域被B型HA的免疫原性区域替换，并且显示这些病毒在小鼠中减毒（J.Virol. 77（2003）803 1）。在这里，我们用这些病毒鼻内免疫小鼠，然后用野生型A病毒攻击它们，以评估对活病毒形式的HA以外的病毒组分的保护性免疫应答。所有免疫小鼠在用致死剂量的野生型病毒攻击后存活;攻击后3天，从小鼠的鼻甲或肺中未回收到病毒或回收到有限量的病毒（如果有的话）。这些结果提供了直接的证据，即对HA以外的病毒组分的免疫应答在小鼠模型中赋予针对甲型流感病毒感染的保护，表明活疫苗对于HA发生抗原漂移的病毒或具有异亚型HA的病毒是有用的。(C)2003爱思唯尔有限公司。保留所有权利。
Previously, we generated influenza A viruses that possess chimeric type (A/B) hemagglutinins (HA), in which immunogenic regions of type A HA were replaced with those of type B HA, and showed that these viruses were attenuated in mice (J. Virol. 77 (2003) 803 1). Here, we intranasally immunized mice with these viruses and then challenged them with a wild-type A virus to assess a protective immune response to viral components other than HA in the form of a live virus. All immunized mice survived challenge with a lethal dose of wild-type virus; none or a limited amount of virus, if any, was recovered from nasal turbinates or lungs of the mice 3 days post-challenge. These results provide direct evidence that immune responses to viral components other than HA confer protection against influenza A virus infection in a mouse model, suggesting the usefulness of live vaccines for viruses that have undergone antigenic drift with respect to HA, or for viruses with heterosubtypic HAs. (C) 2003 Elsevier Ltd. All rights reserved.