An oligonucleotide-ligation assay for the differentiation between Cyclospora and Eimeria spp. polymerase chain reaction amplification products
An oligonucleotide-ligation assay for the differentiation between Cyclospora and Eimeria spp. polymerase chain reaction amplification products
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DOI：
10.4315/0362-028x-62.6.682
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发表时间：
1999-06-01
影响因子：
2
通讯作者：
Wekell, MM
中科院分区：
文献类型：
--
作者：
Jinneman, KC;Wetherington, JH;Wekell, MM
建立了环孢子虫和艾美耳球虫18S rRNA基因PCR扩增产物的寡核苷酸连接法（OLA），并与限制性片段长度多态性（RFLP）检测方法进行了比较。各菌株的正、负分光光度比（A(490)）为4.086 ~ 15.280，中位数为9.5。PCR/OLA为PCR/RFLP提供了一种快速、可靠的分光光度测定方法。
An oligonucleotide-ligation assay (OLA) was developed and compared to a restriction fragment length polymorphism (RFLP) test for distinguishing between 294-bp polymerase chain reaction (PCR) amplification products of the 18S rRNA gene from Cyclospora and Eimeria spp. The PCR/OLA correctly distinguished between three Cyclospora, three E. tenella, and one E. mitis strains and the ratio of positive to negative spectrophotometric absorbance (A(490)) values for each strain ranged from 4.086 to 15.280 (median 9.5). PCR/OLA provides a rapid, reliable, spectrophotometric alternative to PCR/RFLP.