Antigen-specific serotyping of Neisseria gonorrhoeae: characterization based upon principal outer membrane protein
Antigen-specific serotyping of Neisseria gonorrhoeae: characterization based upon principal outer membrane protein
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淋病奈瑟菌的抗原特异性血清分型：基于主要外膜蛋白的表征
DOI：
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发表时间：
1981
影响因子：
3.1
通讯作者：
J. Hildebrandt
中科院分区：
文献类型：
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作者：
T. Buchanan;J. Hildebrandt
淋病奈瑟氏菌的主要外膜蛋白（蛋白I）几乎不含脂多糖（LPS），并通过在pH 9.0的脱氧胆酸盐存在下在Sepharose 6B上对部分纯化的淋球菌外膜进行层析，基本上从其他膜蛋白中纯化出来。将九种不同抗原类型的蛋白I包被到聚苯乙烯管上，并用于酶联免疫吸附测定（ELISA）以测量针对蛋白I的抗体或用于抑制试验以定量蛋白I抗原。来自用于制备每种蛋白I类型的菌株的纯化LPS或带有与用于产生给定蛋白I抗原的菌株相同的LPS但不同的蛋白I抗原的完整淋球菌对ELISA试验没有产生显著的抑制。在125株淋球菌中，有124株（99%）被分为9种蛋白I型中的一种或多种，其中35%被分为单一蛋白I血清型。来自西雅图和亚特兰大的65株播散性淋球菌感染的菌株中有61株（94%）含有蛋白I血清型1，来自西雅图的输卵管炎患者的24株菌株中有16株（64%）携带蛋白I血清型1和2中的一种或两种。
Principal outer membrane protein (protein I) of Neisseria gonorrhoeae was prepared nearly free of lipopolysaccharide (LPS) and substantially purified from other membrane proteins by chromatography of partially purified gonococcal outer membranes over Sepharose 6B in the presence of deoxycholate at pH 9.0. This protein I of nine separate antigenic types was coated to polystyrene tubes and used in the enzyme-linked immunosorbent assay (ELISA) to measure antibody to protein I or in inhibition tests to quantitate protein I antigen. No significant inhibition of the ELISA test was produced by purified LPS from the strain used to prepare each of the protein I types or by whole gonococci bearing the same LPS but different protein I antigens as the strain used to produce a given protein I antigen. Of 125 strains of gonococci used as whole organisms to inhibit the protein I ELISA, 124 (99%) typed with one or more of the nine protein I types, and 35% of these typed with a single protein I serotype. Sixty-one of 65 (94%) strains from Seattle and Atlanta patients with disseminated gonococcal infection contained protein I serotype 1, and 16 of 24 (64%) strains from Seattle patients with salpingitis bore one or both of protein I serotypes 1 and 2.