Protective Effects of Inosine on Urinary Bladder Function in Rats With Partial Bladder Outlet Obstruction
Protective Effects of Inosine on Urinary Bladder Function in Rats With Partial Bladder Outlet Obstruction
复制标题
肌苷对部分膀胱出口梗阻大鼠膀胱功能的保护作用。
DOI：
10.1016/j.urology.2008.10.032
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发表时间：
2009-06-01
期刊：
影响因子：
2.1
通讯作者：
Yamaguchi, Osamu
中科院分区：
文献类型：
--
作者：
Liu, Fei;Yao, Liping;Yamaguchi, Osamu
目的探讨肌苷对大鼠膀胱出口部分梗阻后膀胱功能障碍、神经密度和氧化损伤的保护作用。第1-4组为膀胱出口梗阻组。第1-3组以100、150或225 mg/kg/d的剂量腹腔注射肌苷，第4组以生理盐水给药。第5组为页岩手术对照组。术后21天，对30只大鼠（每组6只）进行膀胱测压。在剩下的30只大鼠中，（每组6只），切除膀胱，用于对各种刺激的收缩反应，免疫组化检测蛋白基因产物9.5（神经元标记物）和超氧化物歧化酶活性和硫代巴比妥酸还原酶物质水平的评价。结果肌苷给药导致对场刺激和卡巴胆碱的收缩反应的剂量依赖性保护作用，尽管对KCl的保护反应仅限于更大剂量的肌苷。与生理盐水处理组相比，在不同剂量的肌苷处理组中注意到残留体积的剂量依赖性减少。此外，蛋白质基因产物9.5阳性神经密度在盐水处理组中降低，但在肌苷处理（225 mg/kg/d）组中显著增加。与生理盐水治疗组相比，肌苷治疗组在150和225 mg/kg/d时观察到超氧化物歧化酶活性显著增强，硫代巴比妥酸还原酶物质水平显著降低。结论这些结果表明，肌苷对部分膀胱出口梗阻引起的大鼠膀胱功能障碍和氧化损伤具有潜在的保护作用。泌尿学73：1417-1422，2009。(C)2009爱思唯尔公司
OBJECTIVES To evaluate the protective effects of inosine on bladder dysfunction, nerve density, and oxidative damage after partial bladder Outlet obstruction in rats.METHODS A total of 60 adult male Sprague-Dawley rats were divided into 5 groups. Groups 1-4 underwent bladder outlet obstruction. Groups 1-3 were treated with inosine at 100, 150, or 225 mg/kg/d intraperitoneally and group 4 with saline. Group 5 consisted of shale-operated control rats. At 21 days postoperatively, cystometry were performed in 30 rats (6 per group). In the remaining 30 rats (6 per group), the bladders were excised and used for contractile responses to various stimulations, immunohistochemical examination for protein gene product 9.5 (a neuronal marker) and evaluation of superoxide dismutase activity and thiobarbituric acid reductase substance level.RESULTS Inosine administration resulted in dose-dependent protective effects on the contractile responses to both field stimulation and carbachol, although the protective responses to KCl was restricted to a greater dose of inosine. A dose-dependent reduction in residual volume was noted in inosine-treated groups at different dosages compared with the saline-treated group. In addition, the protein gene product 9.5-positive nerve density decreased in the saline-treated group but significantly increased in the inosine-treated (225 mg/kg/d) group. Compared with the saline-treated group, significantly enhanced superoxide dismutase activity and a reduced thiobarbituric acid reductase substance level were observed in the inosine-treated group at 150 and 225 mg/kg/d.CONCLUSIONS These results suggest that inosine has a potential protective effect against partial bladder outlet obstruction-induced bladder dysfunction and oxidative injury in rats. UROLOGY 73: 1417-1422, 2009. (C) 2009 Elsevier Inc.