Joint toxicity of lead and cadmium on the behavior of zebrafish larvae: An antagonism.
Joint toxicity of lead and cadmium on the behavior of zebrafish larvae: An antagonism.
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铅和镉对斑马鱼幼虫行为的联合毒性：拮抗作用。
DOI：
10.1016/j.aquatox.2021.105912
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Meng X
中科院分区：
文献类型：
--
作者：
Liao G;Wang P;Zhu J;Weng X;Lin S;Huang J;Xu Y;Zhou F;Zhang H;Tse LA;Zou F;Meng X
虽然铅（Pb）和镉（Cd）的个体毒性已被深入研究，但其联合毒性对昼夜行为节律发育的影响却知之甚少。因此，我们将斑马鱼暴露于铅和镉，以探讨行为节律的变化和潜在的机制。采用电感耦合等离子体质谱法检测重金属内暴露水平。通过视频跟踪系统监测行为节律。采用定量聚合酶链反应和JTK-Cycle分析方法分析褪黑素相关分子和时钟基因的表达变化。结果表明，复合暴露组的铅、镉蓄积水平均显著低于铅、镉组。暴露于铅减少了自发活动，行为节律被镉破坏，而在共同暴露组的模式显示出对自发活动和行为节律的拮抗作用。铅、镉联合染毒使faanat 1表达节律紊乱，mtnr 1aa、mtnr 1bb表达水平降低，mtnr 1c表达水平升高。镉暴露导致clock 1b、clock 2和cry 1b等生物钟基因表达节律的破坏，而联合暴露组仅clock 2的表达节律被破坏。结果表明，镉暴露引起的行为节律紊乱与某些昼夜节律基因的紊乱有关，而铅暴露只减弱了斑马鱼的自发活动，当铅和镉共同暴露时，对斑马鱼幼体的行为模式有拮抗作用。
Although the individual toxicity of lead (Pb) and cadmium (Cd) was intensively studied, little is known about their joint toxicity on the development of circadian behavioral rhythm. Therefore, we co-exposed zebrafish to Pb and Cd to investigate the alterations of behavioral rhythm and the potential mechanism. Inductively coupled plasma mass spectrometry analysis was used to detect the internal exposure level of heavy metals. The behavioral rhythm was monitored by a video-track tracking system. The changes of gene expression regarding melatonin-related molecules and clock genes were analyzed by quantitative polymerase chain reaction and JTK-Cycle analysis. The results showed that the level of Pb2+and Cd2+accumulated in the co-exposure group were significantly lower than that in the Pb or Cd group. Exposed to Pb reduced the locomotor activity; the behavioral rhythms were disrupted by Cd, while the pattern in the co-exposure group showed an antagonistic effect on locomotor activity and behavioral rhythm. The expression rhythm ofaanat1was disturbed and the expression levels ofmtnr1aaandmtnr1bbwere decreased by co-exposure treatment, butmtnr1cwas increased in Pb and Cd group, respectively. Exposure to Cd caused the disruption of expression rhythm in clock genes, likeclock1b, clock2, andcry1b, while only the rhythm ofclock2was disrupted in the co-exposure group. The results suggest that the behavioral rhythm disruption caused by Cd exposure is associated with the disturbance of certain circadian genes, whereas Pb exposure only abates the locomotor activity; an antagonistic effect on the behavioral pattern when co-exposed zebrafish larvae to Pb and Cd.