Antibiotic-induced changes in Tetranychus truncatus bacterial community alter fecundity, longevity and sex-ratio
Antibiotic-induced changes in Tetranychus truncatus bacterial community alter fecundity, longevity and sex-ratio
复制标题
抗生素引起的截尾叶螨细菌群落的变化改变了其繁殖力、寿命和性别比
DOI：
10.11158/saa.25.9.12
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发表时间：
2020
影响因子：
1.2
通讯作者：
Xiao-Yue Hong
中科院分区：
文献类型：
--
作者：
Kun Yang;Han Chen;Xiao-Li Bing;Xue Xia;Yu-Xi Zhu;Xiao-Yue Hong
摘要节肢动物的细菌群落在其寄主的生长发育中起着重要的作用，如提供营养或抵御寄生虫和病原体。然而，对于主要的农作物害虫--树叶叶蝉，人们对其细菌群落知之甚少。为了解不同浓度的盐酸四环素(5 mg~5g/L)浸泡豆叶后对元宝枫体内细菌群落的影响，研究了不同浓度的四环素浸泡豆叶后对其群落的影响。抗生素处理对元宝鱼的适合度有负面影响，降低了其繁殖力和寿命。然而，我们不能排除生殖力下降是由于抗生素的毒性作用的可能性。16S rRNA基因分析表明，抗生素处理对细菌物种多样性没有显著影响，但显著改变了细菌的相对丰度。此外，抗生素处理也改变了微生物区系的预测功能，最丰富的基因与新陈代谢有关。我们的研究表明，虽然不能完全排除抗生素对蜘蛛的直接毒性，但抗生素引起的细菌群落的变化主要是降低了元宝枫的繁殖力。
Abstract The bacterial communities of arthropods play important roles in the growth and development of their hosts, such as by providing nutrition or protecting against parasites and pathogens. However, for Tetranychus truncatus, a major crop pest, very little is known about its bacterial community. To understand the effects of the bacterial community in T. truncatus, mites were fed bean leaves that had been soaked in different concentrations of tetracycline hydrochloride (5 mg to 5 g per L). Antibiotic treatment had a negative impact on the fitness of T. truncatus, decreasing its fecundity and longevity. However, we could not exclude the possibility that the decrease in fecundity was due to a toxic effect of the antibiotic. 16S rRNA genes analyses showed that the antibiotic treatment did not significantly affect the diversity of bacterial species, but it did significantly change their relative abundances. Furthermore, predicted functions of microbiota were also changed with antibiotic treatment and the most abundant genes were related to metabolism. Our research revealed that the antibiotic induced change of bacterial communities mostly decreased the fecundity of T. truncatus, although we couldn't totally exclude the direct toxin of antibiotics on spider mites.