Network connectivity, mechanical properties and cell adhesion for hyaluronic acid/PEG hydrogels
Network connectivity, mechanical properties and cell adhesion for hyaluronic acid/PEG hydrogels
复制标题
DOI：
10.1016/j.biomaterials.2011.05.044
复制
发表时间：
2011-09-01
期刊：
影响因子：
14
通讯作者：
Tirelli, Nicola
中科院分区：
文献类型：
--
作者：
Ouasti, Sihem;Donno, Roberto;Tirelli, Nicola
该研究旨在探索网络结构对HA/PEG凝胶的机械性能和降解性的影响，并突出杨氏模量和细胞定植与选定的结构之间的关系。采用不同浓度和分子量的透明质酸（HA）基光聚合PEG二丙烯酸酯（PEGDA）水凝胶进行了比较（64和234 kDa）的HA：在PEG网络中含有天然HA的半IPN（I型凝胶）;在PEGDA聚合过程中使用硫醇化HA作为链转移剂获得的共网络（II型凝胶）;从原位制备衍生自硫醇化HA在PEGDA上的迈克尔型加成的大分子单体获得的共网络（III型凝胶）。从流变学性质和酶降解性的比较研究，II型凝胶被选择用于进一步的研究，旨在将其机械性能与细胞铺展联系起来。采用RGD功能化的材料，杨氏模量通过AFM纳米压痕测量，而细胞铺展行为通过监测L929成纤维细胞的形态和代谢活性（MTS测定）进行定量评价。通过揭示增加模量和增加细胞铺展/增殖之间的明确关系，该研究表明了用适当的反应性加工技术微调细胞/材料相互作用的可能性。(C)2011爱思唯尔有限公司保留所有权利。
The study aimed to explore the influence of the network architecture on the mechanical properties and degradability of HA/PEG gels, and to highlight the relationship between Young's modulus and cell colonization with a selected architecture. Three different families of hyaluronic acid (HA)-based photopolymerized PEG diacrylate (PEGDA) hydrogels were compared, using different concentrations and molecular weights (64 and 234 kDa) of HA: semi-IPNs containing native HA in a PEG network (type I gels); co-networks obtained using thiolated HA as chain transfer agent during PEGDA polymerization (type II gels); co-networks obtained from the in situ preparation of a macromonomer derived from the Michael-type addition of thiolated HA on PEGDA (type III gels). From a comparative study of rheological properties and enzymatic degradability, type II gels were selected for a further study aiming to link their mechanical properties to cell spreading. Employing RGD-functionalized materials, Young's moduli were measured via AFM nanoindentation while the cell spreading behavior was quantitatively evaluated by monitoring morphology and metabolic activity (MTS assay) of L929 fibroblasts. By revealing a clear relation between increasing modulus and increasing cell spreading/proliferation, the study showed the possibility to fine tune the cell/material interactions with appropriate reactive processing techniques. (C) 2011 Elsevier Ltd. All rights reserved.