MicroRNA expression profiles in avian haemopoietic cells.
MicroRNA expression profiles in avian haemopoietic cells.
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DOI：
10.3389/fgene.2013.00153
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发表时间：
2013
影响因子：
3.7
通讯作者：
Watson M
中科院分区：
文献类型：
--
作者：
Yao Y;Charlesworth J;Nair V;Watson M
MicroRNAs(MiRNAs)是一种小的、丰富的、非编码的RNAs，它通过以序列特异性的方式干扰mRNA转录的翻译或稳定来调节基因的表达。到目前为止，在鸡基因组中共鉴定出734个前体和996个成熟miRNAs。其中许多miRNAs以细胞类型特有的方式表达，要了解它们的功能需要详细研究它们在不同细胞类型中的表达。我们对从刺激或转化的禽类造血细胞系分离的小RNA群体进行了深度测序，以确定这些重要调控分子在这些生物事件中的表达谱变化。在CD40配体刺激的鸡B细胞中，包括miR-155在内的一些miRNAs的表达发生了显著变化。类似地，禽白血病病毒(ALV)转化的DT40细胞也表现出与幼稚细胞相关的miRNA表达的变化。胚胎干细胞系BP25显示了一组明显上调的miRNAs，其中许多先前被证明参与了胚胎干细胞的发育。最后，鸡巨噬细胞系HD11表现出miRNA谱的变化，其中一些被认为与病毒转导的v-myc转化有关。这项工作首次发表了一系列重要禽类细胞中的microRNA表达目录，并提供了对miRNAs在模式非哺乳动物物种细胞造血谱系中的潜在作用的见解。
MicroRNAs (miRNAs) are small, abundant, non-coding RNAs that modulate gene expression by interfering with translation or stability of mRNA transcripts in a sequence-specific manner. A total of 734 precursor and 996 mature miRNAs have so far been identified in the chicken genome. A number of these miRNAs are expressed in a cell type-specific manner, and understanding their function requires detailed examination of their expression in different cell types. We carried out deep sequencing of small RNA populations isolated from stimulated or transformed avian haemopoietic cell lines to determine the changes in the expression profiles of these important regulatory molecules during these biological events. There were significant changes in the expression of a number of miRNAs, including miR-155, in chicken B cells stimulated with CD40 ligand. Similarly, avian leukosis virus (ALV)-transformed DT40 cells also showed changes in miRNA expression in relation to the naïve cells. Embryonic stem cell line BP25 demonstrated a distinct cluster of upregulated miRNAs, many of which were shown previously to be involved in embryonic stem cell development. Finally, chicken macrophage cell line HD11 showed changes in miRNA profiles, some of which are thought to be related to the transformation by v-myc transduced by the virus. This work represents the first publication of a catalog of microRNA expression in a range of important avian cells and provides insights into the potential roles of miRNAs in the hematopoietic lineages of cells in a model non-mammalian species.