EFFICIENT TRANSFER OF ANTIBODIES INTO MAMMALIAN-CELLS BY ELECTROPORATION
EFFICIENT TRANSFER OF ANTIBODIES INTO MAMMALIAN-CELLS BY ELECTROPORATION
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DOI：
10.1016/0022-1759(94)90400-6
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发表时间：
1994-04-15
影响因子：
2.2
通讯作者：
STRAUSS, M
中科院分区：
文献类型：
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作者：
LUKAS, J;BARTEK, J;STRAUSS, M
描述了适合将抗体引入哺乳动物细胞的电穿孔的优化方案。该方法导致 80% 的细胞摄取可检测量的抗体，并引入 40% 的大量抗体。例如，通过将针对 G1 特异性细胞周期蛋白 D1 的单克隆抗体引入 CV-1 和 MCF7 细胞，可以抑制细胞周期活性（从 G1 期过渡到 S 期）。这种特异性抗体介导的细胞功能抑制不会影响细胞的活力，因为它们在一段时间后从抑制中恢复。在接近显微注射效率的同时，抗体电穿孔的新方案还允许处理生化分析所需的大量细胞。
An optimized protocol for electroporation is described which is suitable for introducing antibodies into mammalian cells. The method results in the uptake of detectable amounts of antibodies in 80% of the cells and in 40% large amounts are introduced. As an example, cell cycle activity (transition from the G1 to S phase) was inhibited by the introduction of monoclonal antibodies against G1-specific cyclin D1 into CV-1 and MCF7 cells. This specific antibody mediated inhibition of cellular function did not affect the viability of the cells since they recovered from the inhibition after some time. While approaching the efficiency of microinjection, the new protocol for electroporation of antibodies additionally permits treatment of the larger number of cells which are required for biochemical analyses.