Synthesis of 2,3-butanediol by Synechocystis sp PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria
Synthesis of 2,3-butanediol by Synechocystis sp PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria
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DOI：
10.1016/j.ymben.2013.09.008
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发表时间：
2013-11-01
影响因子：
8.4
通讯作者：
Hellingwerf, Klaas J.
中科院分区：
文献类型：
--
作者：
Savakis, Philipp E.;Angermayr, S. Andreas;Hellingwerf, Klaas J.
将二氧化碳直接有效地转化为液体能源载体和/或大宗化学品对于现代社会的可持续未来至关重要。在这里，我们描述了集胞藻 (Synechocystis sp) 中 2,3-丁二醇的生产。 PCC6803 表达源自肠道和乳酸菌的异源分解代谢途径。该途径由乙酰乳酸合酶、乙酰乳酸脱羧酶和乙偶姻还原酶组成。当编码这三种酶的基因整合到蓝藻基因组中时，以及当它们被质粒编码时，观察到C(4)产物的水平高达0.72 g/l（相当于8 mmol/L），包括水平为043 g/l（相当于4.7 mmol/L）的2,3-丁二醇生产。进一步的优化研究表明，集胞藻内源性表达显着水平的乙酰乳酸合酶，特别是在细胞二氧化碳供应受限的条件下。可溶性转氢酶或 NADPH 依赖性乙偶姻还原酶的共表达可以驱动工程化途径的最后一步接近完成，从而产生纯的 mesa-2,3-丁二醇。 (C) 2013 Elsevier Inc. 保留所有权利
The direct and efficient conversion of CO2 into liquid energy carriers and/or bulk chemicals is crucial for a sustainable future of modern society. Here we describe the production of 2,3-butanediol in Synechocystis sp. PCC6803 expressing a heterologous catabolic pathway derived from enteric- and lactic acid bacteria. This pathway is composed of an acetolactate synthase, an acetolactate decarboxylase and an acetoin reductase. Levels of up to 0.72 g/l (corresponding to 8 mmol/L) of C(4) products, including a level of 043 g/l (corresponding to 4.7 mmol/L) 2,3-butanediol production are observed with the genes encoding these three enzymes integrated into the cyanobacterial genome, as well as when they are plasmid encoded. Further optimization studies revealed that Synechocystis expresses significant levels of acetolactate synthase endogenously, particularly under conditions of restricted CO2 supply to the cells. Co-expression of a soluble transhydrogenase or of an NADPH-dependent acetoin recluctase allows one to drive the last step of the engineered pathway to near completion, resulting in pure mesa-2,3-butanediol being produced. (C) 2013 Elsevier Inc. All rights reserved