FACTORS INVOLVED IN CONTROL OF TISSUE-SPECIFIC EXPRESSION OF ALBUMIN GENE
FACTORS INVOLVED IN CONTROL OF TISSUE-SPECIFIC EXPRESSION OF ALBUMIN GENE
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DOI：
10.1016/0092-8674(87)90036-5
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发表时间：
1987-08-14
期刊：
影响因子：
64.5
通讯作者：
YANIV, M
中科院分区：
文献类型：
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作者：
CEREGHINI, S;RAYMONDJEAN, M;YANIV, M
控制大鼠白蛋白基因的组织特异性表达的元件位于转录起始位点上游约150 bp内。我们在这里显示的DNA酶I足迹分析，至少有四个不同的因素存在于来自细胞表达白蛋白的提取物与这些序列相互作用。这些因子之一与核因子1（NF 1）密切相关或相同。来自非肝组织或来自不转录白蛋白基因的去分化肝癌细胞的提取物显示不同的DNA-蛋白质相互作用模式。混合实验表明，去分化细胞的变异模式是占主导地位的分化的，这表明非表达细胞含有占主导地位的负作用因子。
Elements controlling tissue-specific expression of the rat albumin gene reside within roughly 150 bp upstream of the transcriptional initiation site. We show here by DNAase I footprinting assays that at least four distinct factors present in extracts derived from cells expressing albumin interact with these sequences. One of these factors is closely related or identical to nuclear factor 1 (NF1). Extracts from nonhepatic tissues or from dedifferentiated hepatoma cells that do not transcribe the albumin gene display a different pattern of DNA-protein interactions. Mixing experiments show that the variant pattern of dedifferentiated cells is dominant over that of differentiated ones, suggesting that nonexpressing cells contain dominant negatively acting factors.