Induction of intra- and extra-cellular phospholipids in the lungs of rats exposed to silica.
Induction of intra- and extra-cellular phospholipids in the lungs of rats exposed to silica.
复制标题
暴露于二氧化硅的大鼠肺部细胞内和细胞外磷脂的诱导。
DOI：
10.1042/bj2330111
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hook,GE
中科院分区：
文献类型：
--
作者：
Dethloff,LA;Gilmore,LB;Brody,AR;Hook,GE
在大鼠肺内注射二氧化硅（200 mg/kg）后28天，检查了肺中磷脂的细胞内和细胞外区室。所有含磷脂的隔室均升高，但细胞内和细胞外肺表面活性物质增加最多。细胞内肺表面活性物质从1.18 +/- 0.65增加到144.9 +/- 53.8增加了123倍，细胞外表面活性物质从1.17 +/- 0.04增加到25.1 +/- 7.1 mg/对大鼠肺。细胞内和细胞外表面活性剂的磷脂组成没有改变响应二氧化硅，除了在两个隔室中的总磷脂酰肌醇的百分比增加了近2倍。肺的磷脂含量从24.9 +/- 4.6增加到268.6 +/- 20.8 mg，细胞内和细胞外表面活性剂分别占总增加量的59.1%和24.6%。这些数据表明，二氧化硅引起的肺磷脂含量的主要增加与肺表面活性物质系统有关。
Intracellular and extracellular compartments of phospholipids in the lungs of rats were examined 28 days after intratracheal injection of silica (200 mg/kg). All compartments containing phospholipids were elevated, but the largest increases were seen in the intracellular and extracellular pulmonary surfactant. Intracellular pulmonary surfactant increased 123-fold from 1.18 +/- 0.65 to 144.9 +/- 53.8 and the extracellular surfactant increased 22-fold from 1.17 +/- 0.04 to 25.1 +/- 7.1 mg per pair of rat lungs respectively. The phospholipid composition of intracellular and extracellular surfactant did not change in response to silica, except for an almost 2-fold increase in the percentage of total phosphatidylinositol in both compartments. The phospholipid content of the lungs increased from 24.9 +/- 4.6 to 268.6 +/- 20.8 mg, with the intracellular and extracellular surfactant accounting for 59.1 and 24.6% of this total increase respectively. These data demonstrate that the major increases in the phospholipid content of the lungs induced by silica is associated with the pulmonary-surfactant system.