Characterization of the mechanical properties of polymeric chromatographic particles by micromanipulation
Characterization of the mechanical properties of polymeric chromatographic particles by micromanipulation
复制标题
DOI：
10.1016/j.chroma.2005.08.014
复制
发表时间：
2005-12-02
影响因子：
4.1
通讯作者：
Sprauer, A
中科院分区：
文献类型：
--
作者：
Müller, E;Chung, JT;Sprauer, A
色谱颗粒不仅应具有理想的表面化学性质，还应具有机械性能。后者决定了颗粒在填充床流体力学条件下的变形，进而决定了压降。了解色谱颗粒的机械性能对于成功设计和操作此类处理设备至关重要。采用一种新的显微操作技术，对不同配方和不同表面改性的单色谱颗粒的机械性能进行了表征。该技术的原理是在两个平行表面之间压缩单个颗粒。同时测量施加在颗粒上的力及其在压缩下的变形。颗粒的范围从30到300 μ m，取决于它们的配方。将来自每个样品的10-30个颗粒压缩至70%的颗粒变形，以获得统计学上代表性的数据。疏水相互作用色谱的颗粒被证明比离子交换或凝胶过滤的树脂更稳定。干燥状态下差异显著，湿润状态下差异较小。水似乎“拉平”树脂机械稳定性的差异。稳定性似乎也取决于水从树脂中释放出来的速度。(c)2005 Elsevier B. V.保留所有权利。
Chromatographic particles should not possess only desirable surface chemical properties but also mechanical properties. The latter determine the deformation of the particles under hydrodynamic conditions of packed beds and further the pressure drop. Understanding the mechanical properties of chromatographic particles is essential to successful design and operation of such processing equipment. The mechanical properties of single chromatographic particles made of different formulations and with different surface modifications were characterized by a novel micromanipulation technique. The principle of this technique is to compress single particles between two parallel surfaces. The force being imposed on the particles and their deformation under compression are measured simultaneously. The particles range from 30 to 300 mu m, depending on their formulation. 10-30 particles from each sample were compressed up to a particle deformation of 70% in order to get statistically representative data. Particles for hydrophobic interaction chromatography were proved more stable than resins for ion exchange or gel filtration. The difference is remarkable in dry state and is less in wet state. Water seems to "level out" the differences in mechanical stability of resins. The stability seems to be also dependent on the speed of water release out of the resins. (c) 2005 Elsevier B.V. All rights reserved.