LOCALIZATION OF IRON WITHIN CENTRIC DIATOMS OF THE GENUS THALASSIOSIRA 1
LOCALIZATION OF IRON WITHIN CENTRIC DIATOMS OF THE GENUS THALASSIOSIRA 1
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DOI：
10.1111/j.1529-8817.2012.01165.x
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发表时间：
2012-05
影响因子：
2.9
通讯作者：
Jochen Nuester;S. Vogt;B. Twining
中科院分区：
文献类型：
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作者：
Jochen Nuester;S. Vogt;B. Twining
中心硅藻的细胞铁（Fe）配额已被证明是响应于周围溶解的Fe浓度而变化的，然而，目前尚不清楚中心硅藻如何存储过量的细胞内Fe。在这里，我们使用同步辐射X射线荧光（SXRF）元素映射，以确定铁存储功能的海链藻和海链藻weissfloprogens G。A. Fryxell et Hasle在低和高Fe浓度下生长。局部细胞内铁储存特征，定义为在相对低磷（P），硫（S），硅（Si）和锌（Zn）的区域中具有过高的铁浓度，在T.与低Fe细胞相比，在高Fe条件下生长的Weissflorescent细胞。在高铁条件下，该菌株的细胞Fe配额增加了2.9倍，特征的空间范围增加了4.6倍，特征的Fe含量增加了14倍，这与液泡储存机制一致。Fe特征的元素化学计量与聚磷酸盐结合的Fe作为潜在的空泡Fe储存池一致。铁配额增加了2.5倍，T。在高Fe条件下，Escherichia生长，但与低Fe细胞相比，储存特征仅含有2倍多的Fe，并且尺寸没有增加。T. Chaanana和T. Weissfloprotein可能是由于培养物的生长状态的差异。
The cellular iron (Fe) quota of centric diatoms has been shown to vary in response to the ambient dissolved Fe concentration; however, it is not known how centric diatoms store excess intracellular Fe. Here, we use synchrotron X‐ray fluorescence (SXRF) element mapping to identify Fe storage features in cells of Thalassiosira pseudonana Hasle et Heimdal and Thalassiosira weissflogii G. A. Fryxell et Hasle grown at low and high Fe concentrations. Localized intracellular Fe storage features, defined as anomalously high Fe concentrations in regions of relatively low phosphorus (P), sulfur (S), silicon (Si), and zinc (Zn), were twice as common in T. weissflogii cells grown at high Fe compared to low‐Fe cells. Cellular Fe quotas of this strain increased 2.9‐fold, the spatial extent of the features increased 4.6‐fold, and the Fe content of the features increased 14‐fold under high‐Fe conditions, consistent with a vacuole storage mechanism. The element stoichiometry of the Fe features is consistent with polyphosphate‐bound Fe as a potential vacuolar Fe storage pool. Iron quotas increased 2.5‐fold in T. pseudonana grown at high Fe, but storage features contained only 2‐fold more Fe and did not increase in size compared to low‐Fe cells. The differences in Fe storage observed between T. pseudonana and T. weissflogii may have been due to differences in the growth states of the cultures.