3D imaging of human cells using PIXEμCT

3D imaging of human cells using PIXEμCT
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使用 PIXEμCT 对人体细胞进行 3D 成像

DOI:
10.1142/s0129083514500089
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发表时间:
2014
期刊:
International Journal of PIXE
影响因子:
--
通讯作者:
R. Ortega
R. Ortega
中科院分区:
--
文献类型:
--
作者:
S. Matsuyama;K. Ishii;S. Toyama;K. Watanabe;S. Koshio;K. Kasahara;S. Ito;A. Terakawa;M. Fujiwara;Y. Suzuki;A. Carmona;Stéphane Roudeau;R. Ortega

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我们报告了使用三维(3D)粒子诱导X射线发射微计算机断层扫描(PIXEμCT)对暴露于氧化钴(Co 3 O 4)微粒的人肺上皮细胞进行成像。通过质子微束轰击产生的能量可选择的准单色低能X射线的使用导致了高质量的图像。我们还进行了二维(2D)micro-PIXE成像。3D PIXEμCT成像数据与2D micro-PIXE图像具有互补性,细胞的CT值比值显示Co 3 O 4的强烈吸收。2D micro-PIXE分析提供了元素分布的投影图像,这与3D PIXEμCT成像相结合,揭示了Co 3 O 4内部分布的细节,从而提供了通过细胞内核周积聚的Co 3 O 4毒性机制的见解。
We report imaging of human lung epithelial cells exposed to cobalt oxide (Co3O4) microparticles using three-dimensional (3D) particle-induced X-ray emission microcomputed tomography (PIXEμCT). The use of energy-selectable quasi-monochromatic low-energy X-rays generated via proton microbeam bombardment led to high-quality images. We also carried out two-dimensional (2D) micro-PIXE imaging. The 3D PIXEμCT imaging data are complementary with 2D micro-PIXE images and the CT value ratios of the cells show that the strong absorption stems from Co3O4. The 2D micro-PIXE analysis provides projection images of the elemental distribution, and this in combination with the 3D PIXEμCT imaging revealed details of the internal distribution of Co3O4, and hence provides insight into the mechanisms of Co3O4 toxicity via intracellular perinuclear accumulation.
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发表时间: 2006-08
影响因子: 1.3
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