3D imaging of human cells using PIXEμCT
3D imaging of human cells using PIXEμCT
复制标题
使用 PIXEμCT 对人体细胞进行 3D 成像
DOI:
10.1142/s0129083514500089
复制
发表时间:
2014
期刊:
影响因子:
--
通讯作者:
R. Ortega
中科院分区:
文献类型:
--
作者:
S. Matsuyama;K. Ishii;S. Toyama;K. Watanabe;S. Koshio;K. Kasahara;S. Ito;A. Terakawa;M. Fujiwara;Y. Suzuki;A. Carmona;Stéphane Roudeau;R. Ortega
We report imaging of human lung epithelial cells exposed to cobalt oxide (Co3O4) microparticles using three-dimensional (3D) particle-induced X-ray emission microcomputed tomography (PIXEμCT). The use of energy-selectable quasi-monochromatic low-energy X-rays generated via proton microbeam bombardment led to high-quality images. We also carried out two-dimensional (2D) micro-PIXE imaging. The 3D PIXEμCT imaging data are complementary with 2D micro-PIXE images and the CT value ratios of the cells show that the strong absorption stems from Co3O4. The 2D micro-PIXE analysis provides projection images of the elemental distribution, and this in combination with the 3D PIXEμCT imaging revealed details of the internal distribution of Co3O4, and hence provides insight into the mechanisms of Co3O4 toxicity via intracellular perinuclear accumulation.
DOI:
10.1016/j.nimb.2006.03.127
发表时间:
2006-08
影响因子:
1.3
作者:
K. Ishii;S. Matsuyama;H. Yamazaki;Yutaka Watanabe;Y. Kawamura;T. Yamaguchi;G. Momose;Y. Kikuchi;A. Terakawa;W. Galster
通讯作者:
K. Ishii;S. Matsuyama;H. Yamazaki;Yutaka Watanabe;Y. Kawamura;T. Yamaguchi;G. Momose;Y. Kikuchi;A. Terakawa;W. Galster
DOI:
--
发表时间:
2007
期刊:
International Journal of PIXE 17
影响因子:
--
作者:
H. Yamazaki;S. Matsuyama;et. al.;Y. Kawamura et. al.
通讯作者:
Y. Kawamura et. al.
DOI:
--
发表时间:
2008
期刊:
Proc. the 20th Workshop on Tandem Accelerators and Related Technologies JAEA-Conf 2008-005
影响因子:
--
作者:
Jun;Hasegawa;Shigeki;Shiba;Hitoshi;Fukuda;Yoshiyuki;Oguri
通讯作者:
Oguri