Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
Effects of cocoa extract on glucometabolism, oxidative stress, and antioxidant enzymes in obese-diabetic (Ob-db) rats
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DOI：
10.1021/jf8015915
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发表时间：
2008-09-10
影响因子：
6.1
通讯作者：
Kamaruddin, Syed Hasbullah Syed
中科院分区：
文献类型：
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作者：
Jalil, Abbe Maleyki Mhd;Ismail, Amin;Kamaruddin, Syed Hasbullah Syed
在本研究中，我们研究了可可粉中含有多酚和甲基黄嘌呤的可可提取物对肥胖-糖尿病(Ob-db)大鼠生化指标的影响。肥胖-糖尿病(Ob-db)大鼠采用高脂饮食(脂肪49%，碳水化合物32%，蛋白质19%总能量，千卡)3个月，然后注射低剂量(35 mg/kg体重)链脲佐菌素(STZ)。可可提取物(600 mg/kg体重/d)灌胃4周。结果表明，服用可可提取物4周后，空腹血糖和胰岛素水平无显著差异。口服葡萄糖耐量试验显示，与未补充可可的Ob-db大鼠相比，补充可可的Ob-db大鼠在60分钟和90分钟的血糖显著降低(p<；0.05)。补充可可后，血浆游离脂肪酸和氧化应激生物标志物(8-异前列腺素)显著降低(p<；0.05)。Ob-db组大鼠超氧化物歧化酶活性明显高于未补加组大鼠。而过氧化氢酶活性未见明显变化。结果表明，补充可可对餐后血糖有控制作用，但长期(4周)效果不明显。此外，补充可可可以降低循环血浆游离脂肪酸和8-异前列腺素，并可能增强抗氧化防御系统。
In this present study, we investigated the effects of cocoa extract containing polyphenols and methylxanthines prepared from cocoa powder on the biochemical parameters of obese-diabetic (Ob-db) rats. Obese-diabetic (Ob-db) rats were developed using a high-fat diet (49% fat, 32% carbohydrate, and 19% protein from total energy, kcal) for 3 months, followed by a low dose (35 mg/kg body weight) streptozotocin (STZ) injection. Cocoa extract (600 mg/kg body weight/day) was given to the rats for 4 weeks. The results indicated that there were no significant differences in fasting plasma glucose and insulin level after 4 weeks of cocoa extract administration. Oral glucose tolerance test revealed that cocoa supplementation in Ob-db rats significantly (p < 0.05) reduced plasma glucose at 60 and 90 min compared to unsupplemented Ob-db rats. Plasma free fatty acid and oxidative stress biomarker (8-isoprostane) were significantly (p < 0.05) reduced after cocoa supplementation. Superoxide dismutase activity was enhanced in Ob-db compared to that in nonsupplemented rats. However, no change was observed in catalase activity. The results showed that cocoa supplementation had an effect on postprandial glucose control but not for long term (4 weeks). Moreover, cocoa supplementation could reduce circulating plasma free fatty acid and 8-isoprostane and may enhance the antioxidant defense system.