DELINEATION OF A CROSS-REACTIVE IDIOTYPE ON HUMAN AUTOANTIBODIES WITH ANTIBODY AGAINST A SYNTHETIC PEPTIDE
DELINEATION OF A CROSS-REACTIVE IDIOTYPE ON HUMAN AUTOANTIBODIES WITH ANTIBODY AGAINST A SYNTHETIC PEPTIDE
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DOI：
10.1084/jem.159.5.1502
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发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
CARSON, DA
中科院分区：
文献类型：
--
作者：
CHEN, PP;FONG, S;CARSON, DA
用合成的与第2互补决定区（CDR）对应的肽段（PSL2）和.kappa的邻近氨基酸残基免疫家兔，诱导抗人igm -类风湿因子（RF）抗体交叉反应独特型（CRI）的抗体。IgM-Rf Sie的L链。该抗肽抗体能有效结合已知具有交叉反应独特型的IgM-RF蛋白及其分离的.kappa。链。抗cri被肽- sepharose亲和柱吸收并洗脱。抗体活性被溶液中的游离肽抑制。该抗肽抗体在人IgM-RF上鉴定出一个公共独特型，这在很大程度上取决于L链第2个CDR的初级序列。这种具有预定义特异性的肽诱导的抗独特型可能有助于研究独特型交叉反应的分子基础、抗体分子的遗传和体细胞多样化以及独特型网络的调控。
Antibody against a cross-reactive idiotype (CRI) on human IgM-rheumatoid factor (RF) antibodies was induced by immunization of rabbits with a synthetic peptide (PSL2) corresponding to the 2nd complementarity-determining region (CDR), and adjacent amino acid residues of the .kappa. L chain of the IgM-Rf Sie. The anti-peptide antibody bound efficiently to IgM-RF proteins known to share a cross-reactive idiotype, and to their isolated .kappa. chains. The anti-CRI was absorbed by, and eluted from, a peptide-Sepharose affinity column. The antibody activity was inhibited by the free peptide in solution. The antipeptide antibody identifies a public idiotype on human IgM-RF, that is largely dependent on the primary sequence of the 2nd CDR of the L chain. Such peptide-induced antiidiotypes of predefined specificity may facilitate studies of the molecular basis of idiotypic cross-reactions, the inheritance and somatic diversification of antibody molecules and the regulation of the idiotypic network.