Effects of Peripheral Blood Mononuclear Cells Morphology on Vascular Calcification in Uremic Patients on Maintenance Hemodialysis
Effects of Peripheral Blood Mononuclear Cells Morphology on Vascular Calcification in Uremic Patients on Maintenance Hemodialysis
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DOI：
10.1111/j.1744-9987.2011.01044.x
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发表时间：
2012-04-01
影响因子：
1.9
通讯作者：
Yang, Xueheng
中科院分区：
文献类型：
--
作者：
Chen, Danyan;Gan, Hua;Yang, Xueheng
我们使用高分辨率原子力显微镜（AFM）检查外周血单个核细胞（PBMC）的形态可能发生的变化，并探讨其对维持性血液透析（MHD）尿毒症患者血管钙化的影响。36例MHD后有心血管疾病的尿毒症患者（MHD组1），30例MHD后无心血管疾病的尿毒症患者（MHD组2），20例健康志愿者为对照组。采用冠状动脉钙化评分（CACS）评价冠状动脉钙化程度。AFM用于分析PBMC的细微差别。ELISA法检测PBMC培养上清中骨形态发生蛋白-2（BMP-2）的含量。Western blot检测BMP-2蛋白表达。通过光学显微镜观察，各组间PBMC形态无显著差异。AFM图像显示，尿毒症患者与健康志愿者相比，PBMC形态和血管钙化有显著差异。尿毒症患者PBMCs体积、平均高度、半最大振幅、平均粗糙度、BMP-2和CACS的浓度和表达均高于对照组（P < 0.05），细胞表面有大小不一的颗粒状突起或小窝。MHD 1组与MHD 2组间差异亦有显著性（P < 0.05）。PBMC体积、平均高度、半最大振幅和平均粗糙度与BMP-2和CACS呈正相关。PBMC与BMP-2的相关性高于与CACS的相关性。MHD患者外周血单个核细胞形态与血管钙化程度有关。平均高度、半最大振幅、平均粗糙度和细胞体积越大，血管钙化程度越高。
We used high-resolution atomic force microscopy (AFM) to examine possible changes in the morphology of peripheral blood mononuclear cells (PBMCs), and to investigate their influence on vascular calcification in uremic patients on maintenance hemodialysis (MHD). 36 uremic patients had cardiovascular diseases after MHD (MHD group1) and 30 uremic patients did not (MHD group 2), and 20 healthy volunteers were the control group. The extent of coronary artery calcification was assessed with coronary artery calcification score (CACS). AFM was used to analyze PBMCs nuances. Concentrations of bone morphogenetic protein-2 (BMP-2) in PBMC supernatants were detected by ELISA. Protein expressions of BMP-2 were measured by Western blot. No significant differences in PBMC morphology were observed among groups by light microscopy. AFM images revealed that uremic patients exhibited significant differences of PBMC morphology and vascular calcification when compared with healthy volunteers. The PBMCs in uremic patients were larger in volume, mean height, half-maximum amplitude, average roughness and higher concentrations and expression of BMP-2 and CACS (P < 0.05), with granular processes or caveolae of uneven size distributed over cell surfaces. These differences were also significant between MHD group 1 and group 2 (P < 0.05). PBMC volume, mean height, half-maximum amplitude, and average roughness were positively correlated with BMP-2 and CACS. Moreover, the correlation PBMC with BMP-2 was higher than with CACS. PBMC morphology in MHD patients was related to the degree of vascular calcification. The larger mean height, half-maximum amplitude, average roughness and cell volume were, the higher degree of vascular calcification was.