Supplementation with galactooligosaccharides and inulin increases bacterial translocation in artifically reared newborn rats
Supplementation with galactooligosaccharides and inulin increases bacterial translocation in artifically reared newborn rats
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DOI：
10.1203/pdr.0b013e3181732381
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发表时间：
2008-07-01
影响因子：
3.6
通讯作者：
Darmaun, Dominique
中科院分区：
文献类型：
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作者：
Barrat, Emmanuel;Michel, Catherine;Darmaun, Dominique
添加益生元的配方食品可促进婴儿体内乳酸菌的生长，以及粪便中乳酸盐和乙酸盐的水平。然而，肠腔中高浓度的有机酸已被证明会损害肠屏障功能。为了确定用益生元刺激结肠微生物代谢是否会损害新生儿肠道屏障功能，从出生后第7天（d 7）至断奶（d20），人工饲养的大鼠喂食含有或不含低聚半乳糖/菊粉（GOS/菊粉，88/12; 5.6 g/L）混合物的配方奶粉。在第18天，GOS/菊粉补充增加了结肠腔中乙酸盐和乳酸盐的浓度。尽管回肠相关微生物群和结肠通透性（在Ussing室中评估）以及紧密连接claudin-2和claudin-3 mRNA的表达都没有改变，但GOS/菊粉补充与向脾脏的细菌易位（BT）增加有关。这些影响在d40时均不持续。我们的结论是，GOS/菊粉补充可能会增加BT在一个不成熟的肠道。BT的潜在感染风险与其对新生儿免疫系统成熟的推定有益作用之间的平衡显然值得进一步研究。
Supplementation of formulas with prebiotics enhances the growth of lactate producing bacteria, and fecal lactate, and acetate levels in infants. High concentrations of organic acids in intestinal lumen have, however, been shown to impair the intestinal barrier function. To determine whether stimulating the colonic microbiotal metabolism with prebiotics would impair the neonatal intestinal barrier function, artificially reared rats were fed milk formula with or without a mixture of galactooligosaccharides/inulin (GOS/Inulin, 88/12; 5.6 g/L) from the 7th d of life (d7) until weaning (d20). At d18, GOS/inulin supplementation had increased the concentrations of acetate and lactate in colonic lumen. Although neither ileum-associated microbiota nor colonic permeability (assessed in Ussing chambers), nor the expression of tight junction claudin-2 and claudin-3 mRNA were altered, GOS/inulin supplementation was associated with increased bacterial translocation (BT) toward spleen. None of these effects persisted at d40. We conclude that GOS/inulin supplementation may increase BT in an immature gut. The balance between the potential infectious risk of BT vs. its putative beneficial effect on the maturation of neonatal immune system clearly warrants further study.