Effect of fenpropathrin on the viability and homing ability of worker bees Apis mellifera
Effect of fenpropathrin on the viability and homing ability of worker bees Apis mellifera
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甲氰菊酯对工蜂蜜蜂活力和归巢能力的影响
DOI：
10.1016/j.aspen.2017.08.010
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发表时间：
2017-12-01
影响因子：
1.5
通讯作者：
Wu, Xiao-bo
中科院分区：
文献类型：
--
作者：
Liao, Chun-hua;Wu, Jie;Wu, Xiao-bo
为了探讨甲氰菊酯对蜜蜂存活和归巢能力的影响，将新出现的12h龄蜜蜂工蜂随机分为4组，每组3个重复。将甲氰菊酯(1/2LD50、1/4LD50、1/8LD50和0%LD50)分别注入蜜蜂胸腔。分析工蜂的存活率及其在距蜂群1公里处的归宿率，并对两个记忆相关基因(GluRA和NMDAR 1)在20日龄工蜂中的表达水平进行量化。总体而言，随着甲氰菊酯剂量的增加，小鼠的寿命和归巢率显著降低(P<；0.05)，但最小剂量组(1/8LD50)与对照组(0%LD50)之间差异不显著(P>；0.05)。随着甲氰菊酯剂量的增加，Nmdarl的相对表达显著降低，甲氰菊酯处理组NMDAR 1的表达水平较低。1/8 LD50组和对照组GluRA表达水平显著高于1/2 LD50组和1/4 LD50组(P<；0.05)，1/4 LD50组工人GluRA表达水平显著高于1/2 LD50组(P<；0.05)，1/8 LD50组与对照组比较差异无统计学意义(P>；0.05)。总之，在农作物上使用甲氰菊酯可能会对工蜂的生存能力和归巢能力产生负面影响。
To explore the effect of fenpropathrin on survival and homing ability of honeybees Apis mellifera L., the newly emerged honeybee workers ( < 12 h old) were randomly divided into 4 groups with 3 replicates in each group. Fenpropathrin (1/2 LD50, 1/4 LD50, 1/8 LD50 and 0% LD50) was added on the thorax of the bees. The viability of worker bees and their homing rate at 1 km distance away from colonies were analyzed, and the expression levels of two memory related genes (GluRA and Nmdar 1) in 20-day old worker bees were also quantified. Overall, the lifespan and homing rates were significantly decreased with the increase of fenpropathrin dose (P < 0.05), but there was no significant difference between the least group (1/8 LD50) and the control group (0% LD50) (P > 0.05). The relative expression of Nmdarl was decreased significantly with the increasing doses of fenpropathrin and the lower expression level of Nmdar 1 was found in the fenpropathrin-treaded groups. The expression level of GluRA of workers in 1/8 LD50 group and the control group were significantly higher than that in 1/2 LD50 group and 1/4 LD50 group (P < 0.05), whereas the expression level of GluRA of workers in 1/4 LD50 group was significantly higher than that in 1/2 LD50 group (P < 0.05), and there is no significant difference between 1/8 LD50 group and the control group (P > 0.05). In conclusion, the use of fenpropathrin for agricultural crops may show negative influence on the viability and homing ability of worker bees Apis mellifera L.