Diversity of gut bacterial communities in male adults of Odontolabis fallaciosa (Coleoptera Scarabaeoidea Lucanidae) with different mandibular forms
Diversity of gut bacterial communities in male adults of Odontolabis fallaciosa (Coleoptera Scarabaeoidea Lucanidae) with different mandibular forms
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DOI：
10.16380/j.kcxb.2018.03.007
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发表时间：
2018-03-20
期刊：
影响因子：
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通讯作者：
Wan Xia
中科院分区：
文献类型：
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作者：
Jiang Yu;Sun Bing-Hua;Wan Xia
[目的]分析比较不同下颌型态的成年雄齿齿鱼肠道细菌的群落结构和多样性。[方法]取31例0岁男性成人的肠道组织总DNA。取下颌骨大、中、小三种形态(大下颌骨15例，中下颌骨10例，小下颌骨6例)的下颌骨。用Illumina MiSeq对肠道细菌的16S rDNA基因片段(V3-V4区)进行了测序，并分析了可操作分类单位(OTU)的数量、物种组成、丰度以及α和β多样性。[结果]在97%的相似性阈值下，共获得2238637个高质量的序列，聚为3256个OTU。分析表明，它们可被注明为42门328科542属。其中，4个门(变形杆菌属、拟杆菌属、非杆菌属和细菌门)在雄性成虫肠道细菌群落中占优势地位，4个属(不动杆菌属、异形单胞菌属、巴尔通体属和金杆菌属)为优势种。α多样性分析表明，这些雄鱼样品中肠道细菌丰富，而β多样性分析表明，大、中、小三种雄鱼的OTUS组成不同。[结论]利用Illumina MiSeq测序技术，分析了柳杉成虫肠道细菌群落的结构和组成。大、中下颌骨的成年雄鱼肠道微生物群落组成差异显著，而大、小下颌骨、中、小下颌骨的肠道微生物群落组成差异不显著。
[Aim] To analyze and compare the community structure and diversity of the gut bacteria in adult males of Odontolabis fallaciosa with different mandibular forms. [Methods] The total DNA of guts from 31 male adults of 0. fallaciosa with three mandibular forms (large, medium and small mandible forms) (including 15 adult males of large mandible form, 10 adult males of medium mandible form and 6 adult males of small mandible form) were extracted. The 16S rDNA gene fragments (V3 -V4 region) of gut bacteria were sequenced by Illumina MiSeq, and the number of operational taxonomic units (OTUs), species composition, abundance, and alpha and beta diversity were analyzed. [Results] A total of 2 238 637 high quality sequences were obtained and clustered into 3 256 OTUs at a 97% similarity threshold. Analysis showed that they could be annotated into 42 phyla, 328 families and 542 genera. Among them, four phyla (Proteobacteria, Bacteroidetes, Firmicutes and Tenericutes) were prevalent in the gut bacteria communities of male adults, and four genera (Acinetobacter, Dysgonomonas, Bartonella and Chryseobacterium) were dominant. The alpha diversity analysis showed that the gut bacteria were quite abundant in these male samples of O. fallaciosa, and the beta diversity revealed that the composition of OTUs was different among the large, medium and small mandible males. [Conclusion] Using Illumina MiSeq sequencing technology, the structure and composition of the gut bacterial community in adult males of O. fallaciosa were analyzed. The microbial community composition in guts of adult males of O. fallaciosa has significant difference between the large and medium mandible adult males, whereas no significant difference exists between the large and small mandible adult males, or between the medium and small mandible adult males.