A seed-specific AP2-domain transcription factor from soybean plays a certain role in regulation of seed germination
A seed-specific AP2-domain transcription factor from soybean plays a certain role in regulation of seed germination
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DOI：
10.1007/s11427-008-0044-6
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
Chunmei Wang;Huiwen Wang;Jin‐Song Zhang;S. Chen
中科院分区：
文献类型：
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作者：
Chunmei Wang;Huiwen Wang;Jin‐Song Zhang;S. Chen
植物种子的发育和萌发受到严格的时空调控，转录因子在这一调控中起着重要作用。在本研究中，我们鉴定了一个在发育中的大豆种子中特异性表达的EST。通过进一步的RACE分析获得该基因的全长，并将其命名为gmsgr。序列分析表明该基因属于AP2/ERF转录因子家族。其AP2结构域与拟南芥AP2/ERF家族中DREB亚群A-3成员AtABI4的相似性最高。在酵母试验系统中，GmSGR没有表现出转录激活活性。gmsgr在拟南芥中过表达，在较高浓度的ABA和葡萄糖处理下，转基因种子的发芽率显著高于野生型种子。但在盐胁迫下，转基因种子的发芽率低于对照。atem6和atrd29b在转基因植株幼苗中的表达高于野生型植株幼苗。这些结果表明，GmSGR可能通过调节atem6和atrd29b基因的表达，使转基因种子对ABA的敏感性降低，对盐的敏感性增强。
Plant seed development and germination are under strict temporal and spatial regulation, and transcription factors play important roles in this regulation. In the present study we identified an EST expressed specifically in the developing soybean seeds. The full length of the gene was obtained through further RACE analysis and the gene was namedGmSGR. Sequence analysis revealed that this gene belonged to the AP2/ERF transcription factor family. Its AP2 domain had the highest similarity with that of the A-3 member AtABI4 of DREB subgroup in the AP2/ERF family in Arabidopsis. GmSGR did not exhibit transcriptional activation activity in the yeast assay system.GmSGRwas overexpressed in Arabidopsis and the germination rates of the transgenic seeds were significantly higher than that of the wild type seeds under higher concentrations of ABA and glucose respectively. However, the germination rates of the transgenic seeds were lower than that of control under salt stress. The expression ofAtEm6andAtRD29Bwas higher in the seedlings of the transgenic plants than that in the wild-type seedlings. These results suggest that GmSGR may confer reduced ABA sensitivity and enhanced salt sensitivity to the transgenic seeds through regulating the expression ofAtEm6andAtRD29Bgenes.