Nanoemulsion Therapy for Burn Wounds Is Effective as a Topical Antimicrobial Against Gram-Negative and Gram-Positive Bacteria.
Nanoemulsion Therapy for Burn Wounds Is Effective as a Topical Antimicrobial Against Gram-Negative and Gram-Positive Bacteria.
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DOI：
10.1097/bcr.0000000000000217
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Hemmila MR
中科院分区：
文献类型：
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作者：
Dolgachev VA;Ciotti SM;Eisma R;Gracon S;Wilkinson JE;Baker JR Jr;Hemmila MR
本研究的目的是在啮齿动物体内烫伤模型中研究两种不同纳米乳液制剂对革兰氏阳性菌和革兰氏阴性菌的抗菌功效。将雄性斯普拉格-道利大鼠麻醉并进行部分皮层烫伤。烧伤后八小时，用 1x108 菌落形成单位的铜绿假单胞菌或金黄色葡萄球菌接种伤口。治疗组由两种不同的纳米乳液制剂（NB-201、NB-402）、纳米乳液载体（NE载体）或盐水组成。烧伤后 16 小时和 24 小时进行局部治疗。烧伤后32小时对动物实施安乐死，并获取皮肤样本用于定量伤口培养和真皮炎症标记物的测定。在一组单独的实验中，在治疗 72 小时后通过组织学方法测量烧伤创面的进展情况。与使用生理盐水的动物相比，两种纳米乳液制剂（NB-201、NB 402）均显着减少了铜绿假单胞菌或金黄色葡萄球菌的烧伤创面感染，并将中位细菌计数降低了至少 3 个对数（p<0.0001）。通过髓过氧化物酶测定和组织病理学测量，NB-201 和 NB-402 还减少了真皮中性粒细胞的募集和隔离到伤口中（p<0.05）。此外，促炎性真皮细胞因子（IL-1β、IL-6 和 TNF-α）以及中性粒细胞趋化剂 CXCL1 和 CXCL2 也有所减少。通过组织学检查，NB-201 和 NB-402 似乎都能在受伤后 72 小时抑制烧伤创面进展。局部使用 NB-201 和 NB-402 可有效减少烧伤创面革兰氏阳性菌和阴性菌的生长，减少炎症并阻止烧伤创面的进展。
The aim of this study is to investigate the antimicrobial efficacy of two different nanoemulsion formulations against Gram positive and Gram negative bacteria in an in vivo rodent scald burn model. Male Sprague-Dawley rats were anesthetized and received a partial-thickness scald burn. Eight hours following burn injury the wound was inoculated with 1x108 colony forming units of Pseudomonas aeruginosa or Staphylococcus aureus. Treatment groups consisted of two different nanoemulsion formulations (NB-201, NB-402), nanoemulsion vehicle (NE vehicle), or saline. Topical application of the treatment was performed at 16 and 24 hours after burn injury. Animals were euthanized 32 hours after burn injury and skin samples obtained for quantitative wound culture and determination of dermal inflammation markers. In a separate set of experiments, burn wound progression was measured histologically after 72 hours of treatment. Both nanoemulsion formulations (NB-201, NB 402) significantly reduced burn wound infections with either Pseudomonas aeruginosa or Staphylococcus aureus, and decreased median bacterial counts at least 3 logs as compared to animals with saline applications (p<0.0001). NB-201 and NB-402 also decreased dermal neutrophil recruitment and sequestration into the wound as measured by myeloperoxidase assay and histopathology (p<0.05). In addition, there was a reduction in the pro-inflammatory dermal cytokines (IL-1β, IL-6 and TNF-α) and the neutrophil chemoattractants CXCL1 and CXCL2. By histology examination, both NB-201 and NB-402 appeared to suppress burn wound progression 72 hours after injury. Topically applied NB-201 and NB-402 are effective in decreasing Gram positive and negative bacteria growth in burn wounds, reducing inflammation and abrogating burn wound progression.