Effects of allyl isothiocyanate from horseradish on several experimental gastric lesions in rats
Effects of allyl isothiocyanate from horseradish on several experimental gastric lesions in rats
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DOI：
10.1016/j.ejphar.2006.12.040
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发表时间：
2007-04-30
影响因子：
5
通讯作者：
Yoshikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Matsuda, Hisashi;Ochi, Momotaro;Yoshikawa, Masayuki
众所周知，异硫氰酸烯丙酯是辣根的主要辛辣成分，也是瞬时受体电位（TRP）A1的激动剂。异硫氰酸烯丙酯显著抑制乙醇（1.5 ml/大鼠，p.o.）引起的胃损伤的形成，0.6 M HCl（1.5 ml/大鼠，经口），1%氨（1.5 ml/大鼠，p.o.），和阿司匹林（150 mg/kg，p.o.）(ED50=1.6，2.2，1.7，约6.5 mg/kg，p.o.）。它还显著抑制吲哚美辛（20 mg/kg，p.o.）诱导的胃损伤的形成，虽然抑制作用是ca.高剂量（40 mg/kg，p.o.）时为60%。此外，几种合成的异硫氰酸酯化合物也显着抑制乙醇和吲哚美辛诱导的胃病变。而TRPV 1激动剂辣椒素和胡椒碱则抑制乙醇、1%氨水和阿司匹林引起的胃损伤，但对0.6M HCl引起的胃损伤作用较小。关于作用方式，异硫氰酸烯丙酯对乙醇诱导的胃损伤的保护作用被吲哚美辛预处理减弱，但不与N-G-硝基-L-精氨酸甲酯盐酸盐（L-NAME），或钌红。吲哚美辛预处理降低胡椒碱的保护作用，L-NAME降低辣椒素和奥美拉唑的作用。此外，钌红减少辣椒素，胡椒碱，奥美拉唑的影响。这些结果表明，内源性的洋地黄素发挥了重要作用，烯丙基异硫氰酸酯在乙醇诱导的胃损伤不同的辣椒素，胡椒碱，奥美拉唑的保护作用。(c)2007 Elsevier B. V.保留所有权利。
Allyl isothiocyanate is well known to be a principal pungent constituent of horseradish and an agonist for transient receptor potential (TRP) A1. Ally isothiocyanate markedly inhibited the formation of gastric lesions induced by ethanol (1.5 ml/rat, p.o.), 0.6 M HCl (1.5 ml/rat, p.o.), 1% ammonia (1.5 ml/rat, p.o.), and aspirin (150 mg/kg, p.o.) (ED50=1.6, 2.2, 1.7, ca. 6.5 mg/kg, p.o.). It also significantly inhibited the formation of gastric lesions induced by indomethacin (20 mg/kg, p.o.), though the inhibition was ca. 60% at a high dose (40 mg/kg, p.o.). Furthermore, several synthetic isothiocyanate compounds also significantly inhibited ethanol and indomethacin-induced gastric lesions. Whereas, TRPV1 agonists, capsaicin and piperine, inhibited gastric lesions induced by ethanol, 1% ammonia, and aspirin, but had less of an effect on 0.6 M HCl-induced gastric lesions. With regard to mode of action, the protective effects of ally isothiocyanate on ethanol-induced gastric lesions were attenuated by pretreatment with indomethacin, but not with N-G-nitro-L-arginine methyl ester hydrochloride (L-NAME), or ruthenium red. Pretreatment with indomethacin reduced the protective effects of piperine, and L-NAME reduced the effects of capsaicin and omeprazole. Furthermore, ruthenium red reduced the effects of capsaicin, piperine, and omeprazole. These findings suggest that endogenous prostaglandins play an important role in the protective effect of allyl isothiocyanate in ethanol-induced gastric lesions different from capsaicin, piperine, and omeprazole. (c) 2007 Elsevier B.V. All rights reserved.