A restricted component of the Epstein-Barr virus early antigen complex is structurally related to ribonucleotide reductase.
A restricted component of the Epstein-Barr virus early antigen complex is structurally related to ribonucleotide reductase.
复制标题
EB 病毒早期抗原复合物的限制性成分在结构上与核糖核苷酸还原酶相关。
DOI：
10.1016/0042-6822(87)90331-x
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Pearson,GR
中科院分区：
文献类型：
--
作者：
Goldschmidts,W;Luka,J;Pearson,GR
对先前显示与EBV诱导的早期抗原（EA）复合物的限制性（R）组分相关的85-kDa多肽进行氨基酸测序分析。这是通过溴化氰裂解和通过采用反相C18柱技术的高压液相色谱分离单个肽来完成的。对分离的两种肽F11和F13进行氨基酸测序，发现两者都与B 95 -8株EB病毒的BamH 10右阅读框2（BamH 1 ORF 2）编码的假定蛋白质具有显著的同源性。计算机分析显示，该多肽和核糖核苷酸还原酶的氨基酸序列之间具有显着的同源性，核糖核苷酸还原酶是一种先前映射到该基因组片段的酶。氨基酸组成分析也揭示了类似的关联。这些结果表明，85-kDa EA（R）多肽与EBV诱导的核糖核苷酸还原酶的组分相关。
The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography employing reversed-phase C18column techniques. Two of the isolated peptides, F11 and F13, were subjected to amino acid sequencing and both were found to have significant homology to the postulated protein encoded by theBamHl O right reading frame 2 (BamHl ORF2) of the B95-8 strain Epstein-Barr virus. Computer analysis revealed significant homology between the amino acid sequence of this polypeptide and ribonucleotide reductase, an enzyme previously mapped to this genomic fragment. Amino acid composition analysis also revealed a similar association. These results indicate that the 85-kDa EA(R) polypeptide is associated with a component of the EBV-induced ribonucleotide reductase.