Evaluation of podocyte lesion in patients with diabetic nephropathy: Wilms' tumor-1 protein used as a podocyte marker
Evaluation of podocyte lesion in patients with diabetic nephropathy: Wilms' tumor-1 protein used as a podocyte marker
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DOI：
10.1016/j.diabres.2009.10.022
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发表时间：
2010-02-01
影响因子：
5.1
通讯作者：
Liu, Zhi-Hong
中科院分区：
文献类型：
--
作者：
Su, Jian;Li, Shi-Jun;Liu, Zhi-Hong
简介：肾小球足细胞数量和密度的减少与糖尿病肾病（DN）患者蛋白尿的发生和疾病的进展有关。然而，人们认识到，由于足细胞和肾小球结构的复杂性，通过光学显微镜测量足细胞数量相当困难，不适合临床研究。方法：本实验选用WT 1的C端抗体对足细胞核进行染色，用WT 1的N端抗体对足细胞胞浆进行染色。入选40例2型糖尿病患者，并通过肾活检分析证实患有DN。根据蛋白尿的程度将DN患者分为三组：微量白蛋白尿（n = 10，30-300 mg/24 h），明显蛋白尿（n = 15，0.5-3.5 g/24 h），蛋白尿严重结果：DN患者足细胞数明显减少（减少30-51%），足细胞数与对照组相比无显著性差异（P> 0.05）。尿蛋白与足细胞密度和数量呈显著负相关。肾小球足细胞胞浆覆盖面积密度在所有DN患者中也显著降低（降低39-80%）。一个显着的负相关关系，观察到的覆盖面积密度和蛋白尿的程度。相关系数（r = -0.85）明显高于蛋白尿与足细胞密度（r = -0.56）和足细胞数（T = -0.36）的相关性。结论：DN患者足细胞损伤早于早期，随着蛋白尿的进展，足细胞损伤更为明显。WT 1染色，用多克隆抗体染色细胞核，用单克隆抗体染色细胞质。是研究足细胞损伤的一种有价值的替代技术。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Introduction: The reduction of podocyte number and density per glomerulus has been linked to the development of proteinuria and the progression of disease in patients with diabetic nephropathy (DN). However, it has been recognized that measurement of podocyte number by light microscope is quite difficult because of the complexity of both podocyte and glomerular structure, which is not suitable for clinical research. in our research institute, we used WT1 as podocyte marker to evaluate the podocyte lesion.Methods: In our experiment, we selected the C-terminal antibody of WT1 to stain the nuclei and the N-terminal antibody of WT1 to stain the cytoplasma of podocytes. Forty patients were enrolled with type 2 diabetes and proven to have DN by renal biopsy analysis. DN patients were classified into three groups based on the degree of proteinuria: microalbuminuria (n = 10, 30-300 mg/24 h), overt proteinuria (n = 15, 0.5-3.5 g/24 h), and heavy proteinuria (n = 15, > 3.5 g/24 h).Results: The results demonstrated that the podocyte number was markedly decreased in patients with DN (30-51% reduction). There was a significant negative correlation between the proteinuria and both podocyte density and number. The cover area density of podocyte cytoplasma in glomerulus was also significantly decreased in all DN patients (39-80% reduction). A significant inverse correlation was observed between the cover area density and the degree of proteinuria. The correlation coefficient (r = -0.85) was much higher than that between proteinuria and podocyte density (r = -0.56) or podocyte number (T = -0.36).Conclusion: In conclusion, podocyte damage occurred in patients with DN, even in the early stage and became more dramatic during the course of proteinuria progression. WT1 staining, using the polyclonal antibody to stain the nuclei and monoclonal antibody to stain the cytoplasma. of podocytes together, is a valuable alternative technique in the study of podocyte injury. (C) 2009 Elsevier Ireland Ltd. All rights reserved.