Intestinal colonization with Candida albicans and mucosal immunity
Intestinal colonization with Candida albicans and mucosal immunity
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DOI：
10.3748/wjg.v10.i14.2124
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发表时间：
2004-07-15
影响因子：
4.3
通讯作者：
Tong, Qing-Ying
中科院分区：
文献类型：
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作者：
Bai, Xiao-Dong;Liu, Xian-Hua;Tong, Qing-Ying
目的：观察白色念珠菌定殖与肠道黏膜分泌IgA （sIgA）的关系。方法：将82只无特定病原体小鼠随机分为对照组和定殖组。将白色念珠菌接种于无特定病原体小鼠后，计数白念珠菌粘附于盲肠和粘膜的数量。BrdU掺入显示Peyer's贴片和固有层淋巴细胞增殖，同时研究Peyer's贴片粘膜sIgA（表面膜）同型开关。免疫组化染色观察固有层内IgA浆细胞的变化。ELISA法检测白色念珠菌特异性IgA抗体。结果：白色念珠菌灌胃小鼠后第3 ~ 14天，白色念珠菌在肠腔内定植和粘附于粘膜的数量急剧减少。白色念珠菌在灌胃后的早期时间点转移到肠系膜淋巴结，在较晚的时间点消失。同时，特异性IgA含量明显增加。固有层淋巴细胞的增殖和分化也增加。结论：首次接种白色念珠菌后，特异性无病原体小鼠肠黏膜免疫中固有层淋巴细胞起重要作用。肠道白色念珠菌数量的减少与肠道黏液中特异性IgA抗体水平的升高有关。
AIM: To observe the relationship between intestinal lumen colonization with Candida albicans and mucosal secretory IgA (sIgA).METHODS: A total of 82 specific-pathogen-free mice were divided randomly into control and colonization groups. After Candida albicans were inoculated into specific-pathogen-free mice, the number of Candida albicans adhering to cecum and mucosal membrane was counted. The lymphocyte proliferation in Peyer's patch and in lamina propria was shown by BrdU incorporation, while mucosal sIgA (surface membrane) isotype switch in Peyer's patch was investigated. IgA plasma cells in lamina propria were observed by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.RESULTS: From d 3 to d 14 after Candida albicans gavaging to mice, the number of Candida albicans colonizing in lumen and adhering to mucosal membrane was sharply reduced. Candida albicans translocation to mesenteric lymph nodes occurred at early time points following gavage administration and disappeared at later time points. Meanwhile, the content of specific IgA was increased obviously. Proliferation and differentiation of lymphocytes in lamina propria were also increased.CONCLUSION: Lymphocytes in lamina propria play an important role in intestinal mucosal immunity of specific-pathogen-free mice when they are first inoculated with Candida albicans. The decreasing number of Candida albicans in intestine is related to the increased level of specific IgA antibodies in the intestinal mucus.