Neurofilaments contain alpha-melanocyte-stimulating hormone (alpha-MSH)-like immunoreactivity.
Neurofilaments contain alpha-melanocyte-stimulating hormone (alpha-MSH)-like immunoreactivity.
复制标题
神经丝含有 α-黑素细胞刺激激素 (α-MSH) 样免疫反应性。
DOI：
10.1073/pnas.80.20.6408
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Kleinschmidt,J
中科院分区：
文献类型：
--
作者：
Dräger,UC;Edwards,DL;Kleinschmidt,J
人们发现，一种抗 α-黑素细胞刺激激素 (α-MSH) 的抗血清含有针对 α-MSH 肽上至少两种类型决定簇的抗体：一种仅存在于游离肽上，另一种则与神经丝共享。小鼠大脑的免疫印迹显示神经丝交叉反应性位于 Mr 140,000 范围内的蛋白质上。抗血清的神经丝交叉反应部分可以用细胞骨架制剂选择性地吸收，这消除了抗血清对视网膜的所有亲和力，但不影响垂体中的标记模式。去乙酰-α-MSH 和促肾上腺皮质激素的吸收似乎表明，与神经丝共享的决定簇并不位于 α-MSH 肽的两端，而是位于两者之间。将使用 α-MSH 抗血清对视网膜进行的免疫组织化学标记与使用针对 Mr 200,000 神经丝的单克隆抗体进行的标记进行比较。在成人视网膜中，α-MSH 样免疫反应性被发现稍微更广泛；最一致的是，它可以在大神经节细胞的细胞体中检测到，而这些细胞的体细胞和近端轴突中不存在重神经丝亚基。在发育中的小鼠大脑中，发现重亚基的表达落后于 Mr 140,000 蛋白的表达 2-3 周。这证实了之前的报道，即与较低亚基相比，高分子量神经丝的分布更受限制和晚期表达。
An antiserum to alpha-melanocyte-stimulating hormone (alpha-MSH) was found to contain antibodies to at least two types of determinants on the alpha-MSH peptide: one is present only on the free peptide, the other is shared with neurofilaments. Immunoblots from mouse brain showed the neurofilament crossreactivity to be located on proteins in the Mr 140,000 range. The neurofilament-crossreactive portion of the antiserum could be selectively absorbed out with a cytoskeletal preparation, which abolished all affinity of the antiserum to the retina but did not affect the labeling pattern in the pituitary. Absorptions with desacetyl-alpha-MSH and corticotropin seemed to indicate that the determinant shared with neurofilaments is not located at either end of the alpha-MSH peptide, but somewhere in between. The immunohistochemical labeling of the retina with the alpha-MSH antiserum was compared to the labeling with monoclonal antibodies against Mr 200,000 neurofilaments. In the adult retina the alpha-MSH-like immunoreactivity was found to be slightly more widespread; most consistently it was detectable in cell bodies of large ganglion cells, whereas the heavy neurofilament subunit was absent from somata and proximal axons of these cells. In the developing mouse brain, expression of the heavy subunit was found to lag 2-3 wk behind expression of the Mr 140,000 proteins. This confirms previous reports of a more restricted distribution and late expression of high molecular weight neurofilaments as compared to the lower subunits.