A SPORULATION-INDUCED SIGMA-LIKE REGULATORY PROTEIN FROM B-SUBTILIS
A SPORULATION-INDUCED SIGMA-LIKE REGULATORY PROTEIN FROM B-SUBTILIS
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DOI：
10.1016/0092-8674(81)90157-4
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
LOSICK, R
中科院分区：
文献类型：
--
作者：
HALDENWANG, WG;LANG, N;LOSICK, R
我们分离了一种称为 8 的 sigma 样调节蛋白，其合成或与枯草芽孢杆菌 RNA 聚合酶的关联在孢子形成过程中被诱导。 u2' 是 RNA 聚合酶的孢子形成特异性成分，因为它在早期阻断孢子形成突变体 (SpoDA) 的酶中不存在。我们已经证明了 a2'-RNA 聚合酶使用来自枯草芽孢杆菌染色体 purA-cysA 区域的克隆营养和孢子形成基因簇作为 DNA 模板进行特异性 RNA 合成。 a2'-RNA 聚合酶的基因识别模式与含有 or'5 或 u3'（存在于枯草芽孢杆菌营养细胞中的 σ 因子种类）的 RNA 聚合酶观察到的模式不同。将纯化的 u2' 添加到核心 RNA 聚合酶的重构实验表明，u2' 直接导致孢子形成 RNA 聚合酶转录特异性的改变。我们认为 u2' 是一种调节蛋白，在孢子形成的早期阶段控制发育基因转录。
We have isolated a sigma-like regulatory protein termed 8 whose synthesis or association with Bacillus subtilis RNA polymerase was induced during spore formation. u2’is a sporulation-specific component of RNA polymerase as it was absent in enzyme from an early-blocked sporulation mutant (SpoDA). We have demonstrated specific RNA synthesis by a2’-RNA polymerase using as a DNA template a cloned cluster of vegetative and sporulation genes from the purA-cysA region of the B. subtilis chromosome. The pattern of gene recognition by a2’-RNA polymerase was distinct from that observed for RNA polymerases containing or’5 or u3’, species of sigma factor that are present in vegetative cells of B. subtilis. A reconstitution experiment in which purified u2’was added to core RNA polymerase demonstrates that u2’was directly responsible for the altered transcriptional specificity of sporulation RNA polymerase. We propose that u2’is a regulatory protein that controls developmental gene transcription at an early stage of spore formation.