The spatiotemporal development of adipose tissue
The spatiotemporal development of adipose tissue
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DOI：
10.1242/dev.067686
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发表时间：
2011-11-15
期刊：
影响因子：
4.6
通讯作者：
Koh, Gou Young
中科院分区：
文献类型：
--
作者：
Han, Jinah;Lee, Jung-Eun;Koh, Gou Young
脂肪组织是一种高度专门化的能量储存结构。脂肪细胞是脂肪组织的实质成分，已知起源于中胚层或神经外胚层；然而，脂肪细胞的发育仍然知之甚少。在这里，我们通过分析小鼠出生后附睾脂肪组织(EAT)来研究脂肪组织的发育。在出生后的前14天，EAT被发现是由非脂肪结构产生的。从出生后第1天(P1)到第4天，EAT由体外缺乏成脂分化能力的多潜能前体细胞组成，可视为处于未确定的状态。然而，从P4EAT分离的祖细胞在体外和体内都是通过细胞与基质和细胞与细胞之间的物理相互作用获得成脂分化能力的。此外，我们还发现，VEGFA阻断或巨噬细胞耗尽导致新生小鼠血管生成障碍会干扰脂肪组织的发育。我们得出的结论是，细胞和基质成分之间的适当相互作用以及适当的血管生成对于脂肪组织的发育是必不可少的。
Adipose tissue is a structure highly specialized in energy storage. The adipocyte is the parenchymal component of adipose tissue and is known to be mesoderm or neuroectoderm in origin; however, adipocyte development remains poorly understood. Here, we investigated the development of adipose tissue by analyzing postnatal epididymal adipose tissue (EAT) in mouse. EAT was found to be generated from non-adipose structure during the first 14 postnatal days. From postnatal day 1 (P1) to P4, EAT is composed of multipotent progenitor cells that lack adipogenic differentiation capacity in vitro, and can be regarded as being in the 'undetermined' state. However, the progenitor cells isolated from P4 EAT obtain their adipogenic differentiation capacity by physical interaction generated by cell-to-matrix and cell-to-cell contact both in vitro and in vivo. In addition, we show that impaired angiogenesis caused by either VEGFA blockade or macrophage depletion in postnatal mice interferes with adipose tissue development. We conclude that appropriate interaction between the cellular and matrix components along with proper angiogenesis are mandatory for the development of adipose tissue.