Ex Vivo Electrochemical pH Mapping of the Gastrointestinal Tract in the Absence and Presence of Pharmacological Agents
Ex Vivo Electrochemical pH Mapping of the Gastrointestinal Tract in the Absence and Presence of Pharmacological Agents
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DOI：
10.1021/acssensors.0c01020
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发表时间：
2020-09-25
期刊：
影响因子：
8.9
通讯作者：
Macpherson, Julie, V
中科院分区：
文献类型：
--
作者：
Rajan, Teena S.;Read, Tania L.;Macpherson, Julie, V
本文报道了在不存在和存在旨在改变酸/碳酸氢盐生产的药理学药物的情况下，使用电化学pH探针对（小鼠）上胃肠道的离体pH谱进行分析。首先使用含有0.5%粘蛋白的胃肠道生物模拟缓冲溶液评估三个pH电极的适用性。其中包括传统的玻璃pH探针，氧化铱（IrOx）涂层电极（都是电位法操作）和醌(表面集成硼掺杂金刚石（BDD-Q）电极（伏安法），I在粘蛋白中，IrOx和玻璃提供代表性pH读数的时间尺度在100秒左右，很可能是由于粘蛋白吸附，而BDD-Q电极则为6秒。由于易碎性和分层（IrOx）问题，玻璃和IrOx pH电极的稳健性也受到了影响；与胃肠道组织接触是实验要求。bdd - q被认为是最合适的。沿着胃肠道、食道(1)、胃(5)和十二指肠(4)进行了10次测量。在只有缓冲液的条件下，bdd - q探针追踪到pH值从食管的中性到胃的酸性，然后上升到十二指肠的碱性。奥美拉唑的存在；一个质子泵，在胃的身体区域显示pH值升高。在褪黑素（一种碳酸氢盐激动剂和酸抑制剂）治疗下，胃和十二指肠的pH值均升高。本研究证明了BDD-QpH电极用于实时离体生物组织测量的多功能性。
Ex vivo pH profiling of the upper gastrointestinal (GI) tract (of a mouse), using an electrocheinical pH probe, in both the absence and presence of pharmacological agents aimed at altering acid/bicarbonate production, is reported. Three pH electrodes were first assessed for suitability using a GI tract biological mimic buffer solution containing 0.5% mucin. These include a traditional glass pH probe, an iridium oxide (IrOx)-coated electrode (both operated potentioinetrically), and a qumone ( surface-integrated boron-doped diainond (BDD-Q) electrode (voltammetric),I In mucin, the time scale for both IrOx and glass to provide a representative pH reading was in the 100's of s, most likely due to mucin adsorption, lin contrast to 6 s with the BDD-Q electrode. Both the glass and IrOx pH electrodes were also compromised on robustness due to fragility and delamination (IrOx) issues; contact with the GI tissue was an experimental requirement. BDD-Qwas deemed the most appropriate. Ten measurements were made lalong the GI tract, esophagus (1), stomach (5), and duodenum (4). Under buffer only conditions, the BDD-Qprobe tracked the pH from neutral in the esophagus to acidic in e stomachan nising o more alkaline e duodenum. n e presence ofomeprazole; a proton pump in or, e body regions ofe stomach exhibited elevated pH levels. Under melatonin treatment (a bicarbonate agonist and acid inhibitor), both the body of the stomach and the duodenum showed elevated pH levels. This study demonstrates the versatility of the BDD-QpH electrode for real-tiane ex vivo biological tissue measurements.