Production of fat-1 transgenic rats using a post-natal female germline stem cell line
Production of fat-1 transgenic rats using a post-natal female germline stem cell line
复制标题
使用产后雌性生殖干细胞系生产 fat-1 转基因大鼠。
DOI：
10.1093/molehr/gat081
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发表时间：
2014-03-01
影响因子：
4
通讯作者：
Wu, Ji
中科院分区：
文献类型：
--
作者：
Zhou, Li;Wang, Lei;Wu, Ji
生殖系干细胞系具有自我更新和分化的能力，已经从小鼠和人的卵巢中建立。在这里，我们建立了一个新的雌性生殖系干细胞（FGSC）线从出生后的大鼠免疫磁性分选脆性，这表明一个正常的核型，高端粒酶活性，和一致的基因表达模式的原始生殖细胞培养1年后。利用体外分化系统，FGSC系可以分化为卵母细胞。分别用绿色荧光蛋白（GFP）和fat-1载体转染FGSCs，将其移植到不育大鼠卵巢内。移植的FGSC进行卵子发生，大鼠与野生型雄性大鼠交配后产生携带GFP或fat-1转基因的后代。转基因效率为27.86- 28.00%，转基因鼠的产生需要2个月。这些发现对生物医学研究和生物技术的潜在应用具有影响。
Germline stem cell lines possess the abilities of self-renewal and differentiation, and have been established from both mouse and human ovaries. Here, we established a new female germline stem cell (FGSC) line from post-natal rats by immunomagnetic sorting for Fragilis, which showed a normal karyotype, high telomerase activity, and a consistent gene expression pattern of primordial germ cells after 1 year of culture. Using an in vitro differentiation system, the FGSC line could differentiate into oocytes. After liposome-based transfection with green fluorescent protein (GFP) or fat-1 vectors, the FGSCs were transplanted into the ovaries of infertile rats. The transplanted FGSCs underwent oogenesis, and the rats produced offspring carrying the GFP or fat-1 transgene after mating with wild-type male rats. The efficiency of gene transfer was 27.86-28.00%, and 2 months was needed to produce transgenic rats. These findings have implications in biomedical research and potential applications in biotechnology.