Human recombinant erythropoietin promotes differentiation of murine megakaryocytes in vitro.
Human recombinant erythropoietin promotes differentiation of murine megakaryocytes in vitro.
复制标题
人重组促红细胞生成素在体外促进小鼠巨核细胞分化。
DOI：
10.1172/jci112796
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Burstein,SA
中科院分区：
文献类型：
--
作者：
Ishibashi,T;Koziol,JA;Burstein,SA
为了确定促红细胞生成素是否影响巨核细胞生成，我们在向无血清小鼠骨髓培养物中添加人重组促红细胞生成素后，测量了乙酰胆碱酯酶（AchE）活性，这是小鼠巨核细胞谱系的标志物。促红细胞生成素显着增加 AchE 活性。此外，当将激素添加到来自巨核细胞集落的 426 个分离的单个巨核细胞的无血清培养物中时，促红细胞生成素诱导这些细胞的直径显着增加。根据对某些巨核细胞在培养期间 DNA 含量增加的可能性的贝叶斯分析，我们估计 61% 的细胞倍性增加。这些数据表明促红细胞生成素的作用并不限于红细胞谱系。图像
To determine if erythropoietin affects megakaryocytopoiesis, we measured acetylcholinesterase (AchE) activity, a marker of the murine megakaryocytic lineage, after the addition of human recombinant erythropoietin to serumless murine bone marrow cultures. Erythropoietin increased AchE activity substantially. Moreover, when the hormone was added to serumless cultures of 426 isolated single megakaryocytes derived from megakaryocytic colonies, erythropoietin induced a significant increase in the diameters of these cells. From a Bayesian analysis of the likelihood that some megakaryocytes increased in DNA content during the culture period, we estimate that 61% of the cells increased in ploidy. These data indicate that the action of erythropoietin is not restricted to the erythroid lineage.Images